Team:Illinois/Protocols

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Protocols

This page describes protocols or includes links to protocols used in our project. Recipes used are also listed in a separate section. Protocols are organized by category.

Standard

  • [http://www.biochem.northwestern.edu/morimoto/research/Protocols/I.%20Prokaryotes/A.%20Bacteriology.pdf Bacterial Culture Basics (media/plate preparation, growth, streaking, and storage)]
  • [http://www.methodbook.net/dna/agarogel.html Agarose Gel Electrophoresis Protocol]
  • [http://www.5prime.com/media/29661/perfectprep%20spin%20mini%20kit%20manual_5prime_1048299_072007.pdf Miniprep Protocol (PerfectPrep Spin Mini Kit)]

Recipes

  • LB Growth Media
    • 1L dH20
    • 10g NaCl
    • 5g yeast extract
    • 10g Bacto-tryptone
  • Agarose Gel
    • 50mL 0.5x TBE buffer
    • Agarose (To determine number of grams to use, multiply volume (50mL) by percentage of gel. For example, use 1.5g of agarose in a 3% agarose gel.)
    • 2.5μL ethidium bromide


Questions about our Wiki page? Please email Dave Korenchan at korench1@illinois.edu.

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