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Team:TorontoMaRSDiscovery/23 August 2009
From 2009.igem.org
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August 23, 2009
- Transfected 4+5, Enc+C (x2) ligations into 1 tube of Calvin's cells (aliquots of 33ul of cells)
- plated Enc transformants onto C plates
- Since there were no Tet plates, 400ul of tetracyclin was spread onto a plain LB plate and dried, before plating 4+5 transformants
- Started overnight cultures of 1+2 and 3+2 in 5ml of LB
