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Contents 1 Assembly of BactoMotor 1.1 Introduction 1.2 Prodecures 1.2.1 Step 1. Installation of Micro-Motor 1.2.2 Step 2. Introduction of Bacteria 1.2.3 Step 3. Action of Directional Controller 1.2.4 Step 4. Removing Excessive Bacteria 1.2.5 Step 5. BactoMotor in Action 1.2.6 Step 6. Using the Speed Controller

Assembly of BactoMotor

Introduction

Our BactoMotor is comprised of three parts – the Micro-Motor; the Directional Controller and the Speed Controller.

As their names indicate, the Micro-Motor is the core part of the Motor, and it is small (micrometer scale). The Directional Controller ensures directional propelling force generated by the bacteria, and therefore directional rotation of the motor. The Speed Controller is used to implement a speed control over the motor.

Each module is designed, engineered and tested seperately, and are functional on their own. After testing and fine-tuning, the parts are brought together to form the final BactoMotor. After final assembly, we would have a micrometer-scale BactoMotor which has unidirectional rotation and speed adjustment controls.

Prodecures

Step 1. Installation of Micro-Motor

The Micro-Motor will be installed into a microchip container (Fig.1a, 1b) with separated chambers. Only one side of the arms of the motor is coated with Biotin (for the Directional Controller Module).

Step 2. Introduction of Bacteria

The Bacteria with both Directional Controller and Speed Controller Modules implemented will be introduced to the Micro-Motor (Fig.2).

Step 3. Action of Directional Controller

Under the effect of the Directional Controller, the Bacteria will bind onto the Micro-Motor directionally. (Fig.3a, 3b)

Step 4. Removing Excessive Bacteria

The extra bacterial cells will be removed by medium flush. (Fig. 4)

Step 5. BactoMotor in Action

As the Bacteria swims, they will push the motor into rotation. (clockwise as demonstrated in Fig. 5a, 5b)

Step 6. Using the Speed Controller

To utilize the Speed Controller Module, an inducer is added to the Microchip containing the Micro-Motor. Increase in concentration of the inducer will lead to an overall increase in rotational speed of the Bacto-Motor. (Fig. 6a, 6b)