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Team:IPN-UNAM-Mexico/Notebook
From 2009.igem.org
As summer proyect our experimental work began at 07 - july - 2009 using 2008 bioparts catalog, unfurtunly it dosen’t work properly, we had to much troubles to take out DNA and transform, E. Coli for this we had to delay and request 2009 catalog.
Our proyect uses 29 bioparts, 12 ligations and diferent strategys to make it functional as follow:
June
July
07-July-2009
We digest the follow biobriks with ECORI (E), SPEI (S), and XBAI (X).
| Biobrick | Enzime restriction |
|---|---|
| R0079 | E, S |
| F1610 | E, X |
| B0034 | E, S |
| C0078 | E, X |
| C0079 | E, X |
| B0015 | E, X |
We propose this restrictions for standar assembly for the digest we use a 17 hours incubation at 37º camera.
Digest Mix:
| ERCO RI - SPEI | Per reaction |
|---|---|
| Plasmidic DNA | 3μl |
| Enzima ECORI | 2μl |
| Enzima SPEI | 2μl |
| Buffer NBE | 2μl |
| BSA | 0.5μl |
| H2O | 10.5μl |
| Total | 20μl |
| ECORI - XBAI | Per reaction |
|---|---|
| Plasmidic DNA | 3μl |
| Enzima ECORI | 2μl |
| Enzima XBAI | 2μl |
| Buffer NBE | 2μl |
| BSA | 0.5μl |
| H20 | 10.5μl |
| Total | 20μl |
August
September
October
