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Team:Purdue/Safety
From 2009.igem.org
Home The Team The Project Parts Submitted to the Registry Modeling Notebook Safety
On this page, we will detail our approach to our project's specific biological issues.
Project Safety Issues
Our project raises several safety concerns, primarily for the researcher and to a small degree, the public. Several of our project components have been designated as Biosafety Level 2 (BSL2).
First, our project uses the tat cell-penetrating peptide, originally found in HIV-1. Tat has the ability to cross cell membranes, and can be used for cellular uptake of assorted molecular cargo, if the cargo is bonded to the peptide. In the past, tat has been used to deliver such products as GFP and DNA to cells. Because of the "infectious" characteristics of the tat peptide, it has been designated at a BSL2. While working with the peptide, the researcher must be careful to cover exposed skin areas (wear gloves and long sleeves, or a lab coat), and wear goggles. Work with the peptide must be in a proper microbiological hood.
Second, the glioblastoma cells we are using come from human cancer tissue. Because the cells are human-derived, they are automatically considered BSL2.
Third, the BV-2 microglial cells we are using are derived from mice. However, these microglia have been immortalized by genetic modification. As a result, they are considered BSL2.
The mammalian culture cell lab we work in is a BSL2 facility with equipment and protocols that follow BSL2 guidelines, minimizing safety concerns for the researcher.
Local Biosafety Review Board
At Purdue University, the organization in charge of biosafety is the Radiological and Environmental Management (REM) Department. They work in cooperation with the Institutional Biosafety Committees. For work with recombinant DNA, a protocol must be reviewed and accepted by them to ensure safety.
Here is a link to REM's website
