Team:Wisconsin-Madison/Sequencing Reaction

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Sequencing Reaction

Mixture

  • 1 uL Big Dye
  • 1.5 uL Big Dye Buffer
  • 10 pmol Fwd Primer
  • 10 pmol Rev Primer
  • 0.1 ug DNA Template

The cycling protocol is in spencer's folder under SEQ - don't change the protocol! It is the same no matter what primer Tm you have.

  • Magnetic Bead Clean-up

Get graduate student in Brian's Lab to take reaction to center for processing.


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