Team:Kyoto/CiC/Notebook/week2

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Contents

Week 1 : Construction of HIV TAT LALLA peptide and 11R LALLA peptide

Monday, September 16

To Do on 24 July

  • Miniprep (HIV TAT LALAA peptide_terminator)
  • Restriction Enzyme Digestion

Results

Miniprep
  • HIV TAT LALAAA_terminator was minipreped.
Digestion
  • Digested with Spe1 and Pst1 / Xba1 and Pst1 and run on a gel.
Sample list
NO.Sample NameEnzyme
11-N-23Spe1, PST1
2HIVTATXba1, PST1



Tuseday, September 11

To Do on 24 July

  • Colony PCR(T7promoter_TAT_terminater)
  • Electrophoresis

Results

Electrophoresis

Kyoto e 090724 1.png

  • Very clear bands were found. (See the gel image in right!)
  • The bands are listed below.
Parts and Plasmid length
3-O-23-A-4
Parts length / bp2837487
Plasmid length / bp32663266

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