August/3 October 2009

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1.colony check

sample no.     no. of colony
   1-14N                0
   1-16I                 0
   1-14N                0 
   1-16I               +++
   2-18F              +++

2.transfromation

sample no.
59 , 58 . 60 , 61 , 62 , 63 , 64 , 65

K204066

Vector		Insert
1-19B	2	2-18F	10
EcoRI	1	EcoRI	1
XbaI	1	SpeI	1
No.2	2	No.2	2
dH₂O	14	dH₂O	6
total	20uL	total	20uL

↓
37°C, 2hr
gel electorisis
gel cut & purification
ligation , O/N

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