Edna Miao's notebook

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9/10/09 I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness: ho46 ho176 ho180 ho236 ho252 ho269 ho270 ho320 ho335

9/9/09 I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness: ho46 ho171 ho173 ho178 ho180 ho181 ho251 ho242 ho239 ho268-ho270 ho240

9/8/09

I froze down ho270 for long term storage.

ho253, 241, 200 got contaminated, and were subsequently tossed out.

9/4/09

I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness: ho46 ho245 ho242 ho168 ho230 ho199 ho194 ho266 ho201 ho203 ho268 ho269

9/3/09

I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness: ho46 ho159 ho176 ho182 ho207 ho209 ho203 ho227 ho237 ho348

I froze down these strains for long term storage: ho268-269

9/2/09

I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness: ho46 ho266 ho267 ho262 ho265

9/1/09

I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness: ho172 ho192 ho196 ho198 ho200 ho197 ho46 ho250 ho249 ho243 ho245 ho248 ho261 ho262 ho264 ho265

Ho260 was contaminated, and ho263 had problems, so we threw both strains and their respective data away.

8/31/09 I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness: ho239-243, 245, 248, 250, 253

I froze down these strains for long term storage: ho266, ho267

8/28/09 I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness: ho239-ho265 ho46

8/27/09

I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness: ho174 ho175 ho168 ho212 ho194 ho211 ho46

I froze down these cells for long term storage: ho239-ho265

8/26/09

I transformed plasmids ho312 and ho313 into HO46 cells.

8/21/09

I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness: ho12 ho351 ho352 ho354 ho356-ho363 ho371 ho372 ho46 ho160 ho194 ho199 ho236 ho230 ho46 from alex

8/20/09

I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness: ho12 ho356-ho363 ho371 ho372

I plated ho12, ho356-363, ho371, ho372 for fruiting bodies.

8/19/09

I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness: ho12 ho351 ho352 ho354

8/17/09

I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness: ho12 ho337-341 ho343 ho344 ho346

I tossed these strains and their subsequent data: ho350 ho353 ho355 ho364 - 370 ho373 because they exhibit phenotype similar to P13K1/2- mutants, an error could have been made in a transformation - transformed into HO2 cells instead of HO12.

8/14/09

I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness: ho46 ho230 ho12 ho337-346 ho310-316 ho321-324

I plated ho46, ho211 for the fruiting bodies assay.

8/13/09

I plated ho12, ho337 - ho346 to check their fruiting bodies

8/12/09

I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness: ho12 ho310 - ho316, ho321-ho324

8/10/09

Fruiting Body Assay:

HO46:39 plaques with fruiting bodies.

HO199:3/14 plaques have small fruiting bodies, with one plaque having an abnormally large set of fruiting bodies.

HO 194:0/26 have anything unusual.

HO21:0/29 plaques have anything unusual.

HO211 needs to be redone - only 11 plaques.

HO230:0/30 plaques have anything unusual.

HO235:0/35 plaques have anything unusual.

HO236:0/29 plaques have anything unusual.

HO12:21 plaques have no fruiting bodies.

HO299:72/90 plaques have fruiting bodies.

For more assays, refer to Ethan Chan's notebook.

8/7/09

I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness: HO12, HO300-HO308

8/6/09

Miniprepped 189-44: 133, 60,50, 92, 97, 352, 354 and 189-46: 60, 353, 354

Digested minipreps with PvuI and ran them on a gel. Sequenced 44-354 and 44-353.

Plated PTEN- mutants for fruiting bodies: HO12,HO310-HO319, HO321-HO325

8/5/2009

Froze down HO321-325 for long term storage.

Resequenced 44-353-2.

8/4/2009 I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness: HO12, HO218-HO222 and HO46, HO235-HO236.

I plated these cells for fruiting bodies: HO46, HO235-HO236, HO194, HO199, HO211, HO212, HO230 HO12,HO229, HO237, HO299

I froze down HO310, HO315-HO318 for long term storage.

8/3/09

Fruiting Body Assay HO12: 79/79 plaques have no fruiting bodies

HO218: 34/39 plaques have small fruiting bodies

HO219: 36/40 plaques have regular fruiting bodies

HO220: 21/29 plaques have 2 small fruiting bodies and one big one, the rest are regular

HO221: 33/36 plaques have small spread out fruiting bodies

HO222: 39/47 plaques have small fruiting bodies with 3 plaques having large fruiting bodies I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness: HO12, HO218 - HO222 and HO46, HO235, HO236

Transformations I Transformed HO221, HO275-HO277, HO279, HO318, HO341, HO360-HO364, HO366, HO367, HO369 into HO12. Duplicates of HO233, HO228, and HO342 were made.

7/30/09

Miniprepped 44-50, 44-60, 44-92, 44-97, 44-352, 44-353, 44-354

Redid Microscopy HO160, 168, 169, 174, 175, 205-207, 211, 212

I froze down HO235-237 for long term storage.

PvuI Digested minipreps

7/28/09

I prepared these cells for microscopy and after filming them, I analyzed their speeds and straightness: HO205-207, 211, 212.

Fruiting Body Assay Results HO207: 0/50 plaques have anything interesting

HO205: 0/32 plaques have anything interesting

HO206: 0/49 plaques have anything interesting

HO46 looked normal as well.

I ligated pHO44 to: pHO92, 97, 50, 60, 352, 353, 354 in storage vector backbone pHO189.

I froze down HO229, HO230 for long term storage.

7/27/09

I plated these strains for Fruiting Body Assay: HO12, HO222, HO220, HO218, HO219, HO221

7/24/09

I plated these strains for Fruiting Body Assay: HO199, HO194, HO212, HO211, HO46.

Microscopy Took videos of HO46, HO179, HO199, HO169, HO194, HO175, HO174, HO178

7/23/09

I froze down HO212, HO211 for long term storage

Microscopy Took videos of HO46, HO199, HO194, HO160, HO179, HO12

I Plated HO207, HO206, HO205 for fruiting body assay.