Biobrick/
Ligation Name |
Description |
Creation Date |
N° usage |
Strain |
Vector |
Quality Control |
J23101(J101) |
constitutive promoter |
May 25 |
1 |
TOP10 |
J61002(Amp) |
|
J23102(J102) |
constitutive promoter |
|
|
|
|
|
J23103(J103) |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
J23104(J104) |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
J23105(J105) |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
J23106(J106) |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
J23110(J110) |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
J23112(J112) |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
J23113(J113) |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
J23114(J114) |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
J23116(J116) |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
J23117(J117) |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
J23118(J118) |
constitutive promoter |
May 25 |
1 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
E0240(GFP3) |
GFP protein generatorRBS-GFP-TT |
May 25 |
5 |
TOP10 |
pSB1A2 |
length ok (PCR)sequence ok (MIT) |
K131009(E2) |
colE2 operon:ceaB-ceiB-Tweak-celB-T (with RBS) |
May 28 |
1 |
TOP10 |
pSB1AK3 |
sequence BAD in prefix,suffix,celB (MIT,BMR) |
K117000(celB) |
colE7 lysis gene |
May 28 |
1 |
TOP10 |
pSB1A2 |
sequence ok (MIT) |
K124017(lysis21) |
Phage Lysis Cassette |
May 28 |
0 |
TOP10 |
pSB1AK3 |
sequence BAD (MIT) |
B0015(TT) |
Double terminator |
May 28 |
2 |
TOP10 |
pSB1AK3 |
sequence ok (MIT) |
I732017 (RBS-lacZ) |
RBS-lacZ |
May 28 |
1 |
TOP10 |
pSB1A2 |
long part (MIT) |
B0030 |
RBS 0.6 |
Jun 4 |
0 |
TOP10 |
pSB1A2 |
sequence ok (MIT,BMR) |
B0031 |
RBS 0.07 |
Jun 4 |
0 |
TOP10 |
pSB1A2 |
sequence ok (MIT) |
B0032 |
RBS 0.3 |
Jun 4 |
0 |
TOP10 |
pSB1A2 |
sequence ok (MIT) |
B0033 |
RBS 0.01 |
Jun 4 |
0 |
TOP10 |
pSB1A2 |
sequence ok (MIT) |
B0034 |
RBS 1 |
Jun 4 |
0 |
TOP10 |
pSB1A2 |
sequence ok (MIT) |
P0440(P04) |
RBS-tetR-TT |
Jun 4 |
1 |
TOP10 |
pSB1A2 |
length ok (PCR)sequence ok (MIT) |
R0040(Ptet) |
Ptet |
Jun 4 |
1 |
TOP10 |
pSB1A2 |
sequence ok (MIT) |
T9002 |
Ptet-RBS-luxR-TT-Plux-RBS-GFP-TT |
Jun 4 |
1 |
TOP10 |
pSB1A3 |
sequence BAD (MIT) but seems to work |
J23100(J100bis) |
constitutive promoter |
Jun 4 |
1 |
TOP10 |
J61002(Amp) |
sequence ok (MIT,BMR) |
J23101(J101bis) |
constitutive promoter |
Jun 4 |
1 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
J23118(J118bis) |
constitutive promoter |
Jun 4 |
1 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
A1 |
J23100(Jun 4)-E0240(Pcon-RBS-GFP-TT) |
Jun 11 |
1 |
TOP10 |
pSB1A2 |
|
A2 |
J23101(Jun 4)-E0240(Pcon-RBS-GFP-TT) |
Jun 11 |
0 |
TOP10 |
pSB1A2 |
|
A3 |
I732017-B0015(RBS-lacZ-TT) |
Jun 12 |
1 |
TOP10 |
pSB1AK3 |
length ok (PCR)sequence ok -long part- (BMR) |
A4 |
P0440-R0040(RBS-tetR-TT-Ptet) |
Jun 11 |
1 |
TOP10 |
pSB1A2 |
length ok (PCR)sequence ok (BMR) |
A5 |
A4-A3(RBS-tetR-TT-Ptet-RBS-lacZ-TT) |
Jun 19 |
0 |
TOP10 |
pSB1AK3 |
sequence ok -long part- (BMR) |
A6 |
J23100(Jun 4)-A4(Pcon-RBS-tetR-TT-Ptet) |
Jun 19 |
1 |
TOP10 |
pSB1A2 |
length ok (PCR)sequence ok (BMR) |
A7 |
J23118(Jun 4)-E0240(Pcon-RBS-GFP-TT) |
Jun 25 |
0 |
TOP10 |
pSB1A2 |
|
R0011(Plac) |
Plac |
Jun 25 |
0 |
TOP10 |
pSB1A2 |
sequence ok (MIT) |
K112808(ENTERO) |
holin-lysozyme-T-Pconweak-antiholin-T (with RBSs) |
Jun 25 |
0 |
TOP10 |
pSB1A2 |
sequence ok -long part- (MIT) |
"
