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Team:Berkeley Wetlab/Passenger: Leucine Zippers
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Leucine Zippers
One potential use of cell surface display is to adhere cells together selectively such that there is structure. However, such a feat is impossible to do in one step. In order to work up to such a goal, the first step of such an endeavor is create a basic system of adhere 2 cells types together. We propse using dimeric leucine zippers to adhere cells to one another. Leucine zippers are short peptides which form alpha helices that compliment each other. Using a GNC4 leucine zipper heterodimers KILR and EILD, we hoped to adhere cells to one another.
Functional Assay: Cell-Cell Adhesion
This assay tests for the functionality of displayed leucine zippers via the leucine zipper's ability to dimerized with each other and, as a result, pull the cells out of solution into a clump.
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