Team:Calgary/Lab/Reporter

• Signalling Circuit
• Reporter Circuit
• Mutant Circuits
• Response Circuit

The purpose of the reporter circuit is to test the functionality of the signalling circuit.

Figure 1. Schematic diagram of reporter circuit. The reporter circuit is used to test whether the signalling circuit is functional. It has been designed and constructed with the qrr4 promoter followed by GFP. In the absence of AI-2, the end product of the signalling cascade is phospho-LuxO, which will bind to the qrr4 promoter and therefore produce fluorescence. In the presence of AI-2 however, the end product of the signalling cascade is LuxO, which will not bind to the qrr4 promoter, and therefore the colonies will not glow.

For an in depth look into the construction and testing of reporter circuit (complete with results) please read the paper below.