Today we started the construction of our first biobrick. After quantificate the miniprep products (from 11/Aug) we performed the digestion (Protocol 14) with SpeI and PstI of plasmids containing the RBS and the CFP reporter in order to connect this parts.
After the digestion we isolated the target bands from the agarose gel, according to the Protocol 7.
The CFP digested band was correctly purified from the gel, unfortunatly we lost the RBS fragment. =( It must have been lost at some stage of the purification, therefore we could not ligate this two parts today.
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