Team:Osaka/NOTES

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NOTES

Calendar

July
MTWTFSS
    [http://2009.igem.org/wiki/index.php?title=July/1_July_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=July/2_July_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=July/3_July_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=July/4_July_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=July/5_July_2009&action=edit 5]
[http://2009.igem.org/wiki/index.php?title=July/6_July_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=July/7_July_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=July/8_July_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=July/9_July_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=July/10_July_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=July/11_July_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=July/12_July_2009&action=edit 12]
[http://2009.igem.org/wiki/index.php?title=July/13_July_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=July/14_July_2009&action=edit 14] [http://2009.igem.org/wiki/index.php?title=July/15_July_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=July/16_July_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=July/17_July_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=July/18_July_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=July/19_July_2009&action=edit 19]
[http://2009.igem.org/wiki/index.php?title=July/20_July_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=July/21_July_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=July/22_July_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=July/23_July_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=July/24_July_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=July/25_July_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=July/26_July_2009&action=edit 26]
[http://2009.igem.org/wiki/index.php?title=July/27_July_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=July/28_July_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=July/29_July_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=July/30_July_2009&action=edit 30] [http://2009.igem.org/wiki/index.php?title=July/31_July_2009&action=edit 31]
August
MTWTFSS
          [http://2009.igem.org/August/1_August_2009 1] [http://2009.igem.org/August/2_August_2009 2]
[http://2009.igem.org/August/3_August_2009 3] [http://2009.igem.org/August/4_August_2009 4] [http://2009.igem.org/August/5_August_2009 5] [http://2009.igem.org/August/6_August_2009 6] [http://2009.igem.org/August/7_August_2009 7] [http://2009.igem.org/August/8_August_2009 8] [http://2009.igem.org/August/9_August_2009 9]
[http://2009.igem.org/August/10_August_2009 10] [http://2009.igem.org/August/11_August_2009 11] [http://2009.igem.org/August/12_August_2009 12] [http://2009.igem.org/August/13_August_2009 13] [http://2009.igem.org/August/14_August_2009 14] [http://2009.igem.org/August/15_August_2009 15] [http://2009.igem.org/August/16_August_2009 16]
[http://2009.igem.org/August/17_August_2009 17] [http://2009.igem.org/August/18_August_2009 18] [http://2009.igem.org/August/19_August_2009 19] [http://2009.igem.org/August/20_August_2009 20] [http://2009.igem.org/August/21_August_2009 21] [http://2009.igem.org/August/22_August_2009 22] [http://2009.igem.org/August/23_August_2009 23]
[http://2009.igem.org/August/24_August_2009 24] [http://2009.igem.org/August/25_August_2009 25] [http://2009.igem.org/August/26_August_2009 26] [http://2009.igem.org/August/27_August_2009 27] [http://2009.igem.org/August/28_August_2009 28] [http://2009.igem.org/August/29_August_2009 29] [http://2009.igem.org/August/30_August_2009 30]
[http://2009.igem.org/August/31_August_2009 31]
September
MTWTFSS
  [http://2009.igem.org/September/1_September_2009 1] [http://2009.igem.org/September/2_September_2009 2] [http://2009.igem.org/September/3_September_2009 3] [http://2009.igem.org/September/4_September_2009 4] [http://2009.igem.org/September/5_September_2009 5] [http://2009.igem.org/September/6_September_2009 6]
[http://2009.igem.org/September/7_September_2009 7] [http://2009.igem.org/September/8_September_2009 8] [http://2009.igem.org/September/9_September_2009 9] [http://2009.igem.org/September/10_September_2009 10] [http://2009.igem.org/September/11_September_2009 11] [http://2009.igem.org/September/12_September_2009 12] [http://2009.igem.org/September/13_September_2009 13]
[http://2009.igem.org/September/14_September_2009 14] [http://2009.igem.org/September/15_September_2009 15] [http://2009.igem.org/September/16_September_2009 16] [http://2009.igem.org/September/17_September_2009 17] [http://2009.igem.org/September/18_September_2009 18] [http://2009.igem.org/September/19_September_2009 19] [http://2009.igem.org/September/20_September_2009 20]
[http://2009.igem.org/September/21_September_2009 21] [http://2009.igem.org/September/22_September_2009 22] [http://2009.igem.org/September/23_September_2009 23] [http://2009.igem.org/September/24_September_2009 24] [http://2009.igem.org/September/25_September_2009 25] [http://2009.igem.org/September/26_September_2009 26] [http://2009.igem.org/September/27_September_2009 27]
[http://2009.igem.org/September/28_September_2009 28] [http://2009.igem.org/September/29_September_2009 29] [http://2009.igem.org/September/30_September_2009 30]
October
MTWTFSS
      [http://2009.igem.org/August/1_October_2009 1] [http://2009.igem.org/August/2_October_2009 2] [http://2009.igem.org/August/3_October_2009 3] [http://2009.igem.org/August/4_October_2009 4]
[http://2009.igem.org/August/5_October_2009 5] [http://2009.igem.org/August/6_October_2009 6] [http://2009.igem.org/August/7_October_2009 7] [http://2009.igem.org/August/8_October_2009 8] [http://2009.igem.org/August/9_October_2009 9] [http://2009.igem.org/August/10_October_2009 10] [http://2009.igem.org/August/11_October_2009 11]
[http://2009.igem.org/August/12_October_2009 12] [http://2009.igem.org/August/13_October_2009 13] [http://2009.igem.org/August/14_October_2009 14] [http://2009.igem.org/August/15_October_2009 15] [http://2009.igem.org/August/16_October_2009 16] [http://2009.igem.org/August/17_October_2009 17] [http://2009.igem.org/August/18_October_2009 18]
[http://2009.igem.org/wiki/index.php?title=August/19_October_2009&action=edit 19] [http://2009.igem.org/August/20_October_2009 20] [http://2009.igem.org/wiki/index.php?title=August/21_October_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=August/22_October_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=August/23_October_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=August/24_October_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=August/25_October_2009&action=edit 25]
[http://2009.igem.org/wiki/index.php?title=August/26_October_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=August/27_October_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=August/28_October_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=August/29_October_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=August/30_October_2009&action=edit 30] [http://2009.igem.org/wiki/index.php?title=August/31_October_2009&action=edit 31]
November
MTWTFSS
            [http://2009.igem.org/wiki/index.php?title=November/1_November_2009&action=edit 1]
[http://2009.igem.org/wiki/index.php?title=November/2_November_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=November/3_November_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=November/4_November_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=November/5_November_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=November/6_November_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=November/7_November_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=November/8_November_2009&action=edit 8]
[http://2009.igem.org/wiki/index.php?title=November/9_November_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=November/10_November_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=November/11_November_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=November/12_November_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=November/13_November_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=November/14_November_2009&action=edit 14] [http://2009.igem.org/wiki/index.php?title=November/15_November_2009&action=edit 15]
[http://2009.igem.org/wiki/index.php?title=November/16_November_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=November/17_November_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=November/18_November_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=November/19_November_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=November/20_November_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=November/21_November_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=November/22_November_2009&action=edit 22]
[http://2009.igem.org/wiki/index.php?title=November/23_November_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=November/24_November_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=November/25_November_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=November/26_November_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=November/27_November_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=November/28_November_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=November/29_November_2009&action=edit 29]
[http://2009.igem.org/wiki/index.php?title=November/30_November_2009&action=edit 30]


Registry Parts

Osaka team ALL PARTS hereParts & Devices

COLOR
K204033 mOrange


placI
K204041 placI+GFP
K204042 placI+YFP
K204043 placI+CFP
K204044 placI+RFP
K204048 placI+mOrange

pcI
K204058 pcI+YFP
K204059 pcI+CFP
K204062 pcI+mOrange


ptetR
K204052 ptetR+mOrange

Carotenoid
K204064 crtE+crtB
K204065 crtI+crtY
K204068 crtZ

K204076 placI+crtE+crtB
K204077 ptetR+crtE+crtB
K204091 placI+crtE+crtB+crtI+crtY
K204095 placI+crtE+crtB+crtZ
K204096 ptetR+crtE+crtB+crtZ

gradation
K204069 cI repressor
K204086 3O6HSL receiver+RFP+cI repressor

SIGNAL (Quorum Sensing)

AI-1
K204047 LasI generater


C4HSL
K204040 RhlI (generater)
K204031 RhlR (receiver)
K204702 RhlR+GFP


3OH,C14 : 1-HSL
K204025 cinI (generater)
K204032 cinR (receiver)


3O6HSL
K204051 LuxR (receiver)
K204700 LuxR+GFP


MOTILITY
K204018 ptetR+EspE

K204501 RBS+FliH
K204502 ptetR+FliH
K204503 pT7+FliH
K204504 C4HSL receiver +FliH


Lab Note

WET Lab


Week 1 : 8/2~8/8
Brainstorming , parts planning , prepared competent cell
transformation and miniprep from MIT plate

week 2 : 8/9~8/15
start assembly parts (K204001 ~ K204006) , transformation and mini prep from MIT

Week 3 : 8/16~8/22
assembly parts (~K204015) , parts planning

Week 4 : 8/23~8/29
assembly parts (~K204021)

Week 5 : 8/30~9/5
assembly parts (~K204030)

Week 6 : 9/6~9/12
assembly parts (~K204046)

Week 7 : 9/13~9/19
assembly parts (~K204050)

Week 8 : 9/20~9/26
assembly parts (~K204056)
TEST : sensitivity of inducer and receptor
Sample : 3O6HSL , C4HSL , AI-1 , 3OH,C14:1-HSL
Result : Not confirm the fluorescence...

Week 9 : 9/27~10/3
assembly parts (~K204066 , X1~X8) , color (fluorescence) intensity check

Week 10 : 10/4~10/10
assembly parts (~K204078)
Lux receiver sensitivity test , color (fluorescence) intensity check
sequence check : generator and receiver
3O6HSL , C4HSL , AI-1 , 3OH,C14:1-HSL
Get the parts from the Chiba University Team. , Thanks chiba team

Week 11 : 10/11~10/17
assembly parts (~K2040104)
sequence check : FliH etc…
send Osaka team parts to MIT
FINISH!
Osaka team assembled about 120 parts!

Dry Lab

Week 5 : 8/30~9/5
Planing model.

Week 6 : 9/6~9/12
Studying programing using Matlab.

Week 7 : 9/13~9/19
Making program in case of single coloney.

Week 8 : 9/20~9/26
Making program in case of more than two colonies
Making movie file
Searching paper written some parameter

Week 9 : 9/27~10/3
Blash up our program (~K204066 , X1~X8) , color (fluorescence) intensity check
Searching paper written some parameter

Week 10 : 10/4~10/10
Making program of color gradation

Week 11 : 10/11~10/17
Blash up our program

etc

Under construction



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