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Team:Nevada/Project
From 2009.igem.org
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The goal of our project is to clone one or more genes from the cynnamaldehyde pathway into E. coli as a potential insecticide against mosquito larvae. The ultimate goal of this project would be to move one or more of these genes into Wolfia, a small aquatic plant to which mosquito larvae feed upon during the spring and summer months. |  |
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Overall project
Your abstract
Project Details
The goal of our project is to clone one or more genes from the cynnamaldehyde pathway into E. coli as a potential insecticide against mosquito larvae. The ultimate goal of this project would be to move one or more of these genes into Wolfia, a small aquatic plant to which mosquito larvae feed upon during the spring and summer months.
Goal 1
The initial goal of this project is to clone the Arabidopsis gene cynnamaldehyde CoA-reductase into an E. coli expression system and test for activity. Currently, we are planning to use an inducible promoter, a RBS, and double terminator from the standard registry of parts to create this construct.
The Experiments
1. Conduct a three way ligation with pBAD (or LacI), RBS and a chloromphenicol resistant plasmid backbone (recombinant 1). 2. Conduct a three way ligation with cynnamaldehyde CoA, double terminator and chloromphenicol resistant plasmid (recombinant 2). 3. Conduct a three way ligation with recombinant 1, 2 and a tetracycline resestant plasmid.
