The redoxilator
- Genetic design
- Applications and perspectives
- Results
- Safety considerations
The USER assembly standard
- USER fusion of biobricks
USER fusion primer design software
- Abstract
- Instructions
- Output format
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The project
Results
Two strains were grown under diferent substrates, Glucose, Galactose, Glucose and Galactose and Ethanol.
These two strains that we denominate Construct and Leaky correspond to the redoxilator with a constitutive promoter and a minimal promoter repectivelly.
Construct
Growth rates (h-1) obtained for the two strains on diferent carbon sources
Carbon Source |
Leaky |
Construct |
Glucose |
0,114 ± 0,006 |
0,112 ± 0,003 |
Galactose |
0,084 ± 0,010 |
0,076 ± 0,003 |
Ethanol |
E0,013 ± 0,005 |
0,007 ± 0,001 |
Achievements
Redox sensing device
Two novel genes have been designed and synthesized each comprised of 5 genetic elements. Together they function as a device termed the Redoxilator that can sense the internal redox state of a yeast cell, and output a reporter signal.
Biobricks
DNA of several new biobricks have been designed and submitted including a yeast optimized GFP reporter protein, a protein degradation sequence and a fast degradable yeast GFP. (Bronze medal)
We have demonstrated that our USER fusion biobrick works as expected and documented it (silver medal)
USER fusion Assembly standard
A new biobrick assembly standard that allows the rapid construction of multi-part devices have been developed and documented. The assembly standard offers many benefits: All restriction sites are allowed, multiple biobricks can be joined in one step, the result is scar-free making it ideal for protein fusions and more. (Gold medal)
USER-fusion primer design software
A novel and very useful software tool have been developed that can automatically design the optimal primers for USER fusion assembly of 2-9 biobricks, taking several parameters into account.
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