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Team:Bologna
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Project Summary
Which is our idea?
The project aims to design a new device to control the synthesis of a protein in ''Escherichia coli'' by acting at translational level regardless of the protein to be controlled. This "general-purpose" standard device could allow a faster silencing of protein expression with respect to standard regulated promoters. Our device was named T-Rex (Trans Repressor of Expression). It consists of two new BioBricks, i.e. the Trans-repressor and the Cis-repressing.
How can we achieve our goal?
T-Rex device consists of two new BioBricks:
CIS-repressing and TRANS-repressor were designed using our [[Team:Bologna/Software#1|BASER]] tool.
Transcription of the target gene produces a mRNA strand, starting with the Cis element, which is translated into proteins by ribosome. Trans’ promoter induction produces a transcript that binds with the Cis part. The RNA duplex prevents ribosome from binding to RBS, repressing protein synthesis. Thus, the TRANS-repressor amount regulates the gene mRNA translation rate.
How can we test the device?
In order to test and characterize our T-REX device, we developed the following genetic circuit:
The T-REX device is proposed as a universal and fast switch in synthetic gene circuits.
More details about our work in the Project section.
Acknowledgements
- [http://www.unibo.it/Portale/default.htm University of Bologna]
- [http://serinar.criad.unibo.it Ser.In.Ar. Cesena]
- Cultural Association San Sebastiano
