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Team:Bologna
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Project Summary
Which is our idea?
The project aims to design a new device to control the synthesis of a protein in Escherichia coli regardless of the protein to be controlled. This "general-purpose" standard device acts on translation to allow a faster silencing of protein expression if compared to standard regulated promoters. We named this device T-Rex (Trans Repressor of Expression).
How does T-Rex work?
The device consists of two new BioBricks:
CIS-repressing and TRANS-repressor were designed using our BASER software.
Transcription of the target gene produces a mRNA strand, starting with the Cis element, which is translated into proteins by ribosome. Trans’ promoter induction produces a transcript that binds with the Cis part. The RNA duplex prevents ribosome from binding to RBS, repressing protein synthesis. Thus, the TRANS-repressor amount regulates the gene mRNA translation rate (Fig 1)
How can we test the device?
In order to test and characterize our T-REX device, we developed the following genetic circuit (Fig 2):
The T-REX device is proposed as a universal and fast switch in synthetic gene circuits.
More details about our work in the Project section.
Acknowledgements
- [http://www.unibo.it/Portale/default.htm University of Bologna]
- [http://serinar.criad.unibo.it Ser.In.Ar. Cesena]
- Cultural Association San Sebastiano
