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Team:LCG-UNAM-Mexico/Resources/Protocols/Ligation
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Revision as of 19:05, 21 October 2009 by Abrahamavelar (Talk | contribs)
To ligate two biobricks into a base vector.
Part 1: 2 µl Part 2: 2 µl Destination Plasmid: 2 µl 10X T4 Ligase Buffer: 2 µl T4 Ligase: 1 µl H2O: 11 µl
Incubate at room temperature for 10 minutes and then inactivate the enzyme at 80°C for 20 minutes.
Transform 5 μl of the ligation product into 50 μl of competent DH5-alpha E. coli cells.
