Team:LCG-UNAM-Mexico/Resources/Protocols/ColonyPCR

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Colony PCR
Take the colony and resuspend in 200uL of TE 10:1 NaCl 10M
Heat the suspension for 10 minutes at 95°C
Centrifugue for 2 minutes at 14000 RPM
Use 10uL of this DNA extract as template for the PCR

The mixture to perform the PCR is:
23uL H2O
2.5uL Forward primer
2.5uL Reverse primer
3.5uL dNTPs
2.5uL MgCl2
5uL Buffer10X
10uL DNA template
1uL taqDNA polymerase.

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