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Team:Heidelberg/Project dual assay plasmid
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which should have the advantage of two fluorescent proteins with different promoters. One of them would be constitutive the other one to be tested. This construction would allow a standardized comparison of promoter strength, due to the elimination of different transfection efficiencies. Unfortunately the repeated cloning of the construct, which is shown in Fig 1, was not successful.
Fig. 1: The planned dual assay plasmid contained a site for promoter exchange, where one can put the promoter, that has to be tested. This promoter is upstream of a GFP gene and regulates its transcription. This gene is followed by a Jet promoter that constitiutively promotes transcription of a mCherry, serving as a reference.
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