The Toluene Terminator Model

Overview :

The following mathematical model examines the dynamics of the suicide mechanism that employed tunable repression. As outlined in the project description, the Pu promoter is placed in front of a repressor system that would inhibit the production of holin and lysozyme, while a constitutive promoter is placed in front of the gene for antiholin. In the presence of toluene, the Pu promoter would be activated, leading to repression of holin/lysozyme production and therefore cell survival. In the absence of toluene, the Pu promoter would not be activated, and as a result holin and lysozyme would be produced, leading to cell death.

Cell Growth/Death

Cell Lysis happens when holin creates pores in membrane and lysozyme enters Assume rate of cell death ∝[holin](# cells), rate of cell growth in exponential phase

Transcription of Repressor :

𝑓([𝑇]) is a function of toluene concentration

Use Hill Equation to describe binding affinity of toluene to Pu promoter.

Transcription of Lysozyme and Holin:

Assume transcription rate is proportional to # of free operator sites (which should be the same for both lysozyme and holin, since they are downstream of the same promoter)

Assume transcription rates for H and L are the same

Repression of Lysozyme and Holin Transcription

Repressor binds with free operator site, preventing transcription of lysozyme and holin. Use a model invoking law of mass action.

Assuming that fops and Rfops do not undergo spontaneous degradation.

Production of Repressor

Using the translation rate for R and taking into consideration the binding of R with fops,

Production of Proteins

Production of antiholin, under constitutive promoter, is at a constant rate γA, which depends on the promoter that is used

Dimerization: Since holin and antiholin form a complex

Using the translation rates and incorporating dimerization using the law of mass action,

Assumptions

Can set γA equal to rate of production of holin in the case that all operating sites are free

• This is in order to balance antiholin and holin levels without repression
• This can be tuned so that timing of cell death works out
• Set antiholin production rate to that of holin in the absence of repression
• Put this through the transcription and translation equations to obtain production rate

Assume degradation rates of all mRNAs are the same

• Can directly search for these rates in literature
• Can use half life to calculate rate

For protein degradation rate:

Case 1: Proteins are stable:

• Degradation rates equal reproduction rate of cell
• This is due to dilution of proteins across daughter cells

Case 2: Proteins are unstable

• If not other degradation rate information is provided, can possibly assume that the degradation rates are a few orders of magnitude above the reproduction rate of cell
• Will have to look into this further

Variables and Parameters

Further Work

We plan to run simulations of this model using MATLAB, focusing on the relationship between cell count and toluene concentration. Before this is done, we must find/estimate values for the parameters listed above. Initial simulations will be tailored to fine-tuning parameters to satisfy the primary needs of the modeling: at high toluene concentrations, the cells will survive; at low toluene concentrations, the cells will die. After that, we will look at low-to-high and high-to-low transitions in toluene concentrations and look for equilibria that arise as a result. This will help us understand how responsive the system is to toluene levels (including initial concentrations).