Team:Chiba/Notebook/Calendar/30 September 2009

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Contents

Transformation

Yesterday's operation is here.


  • Today's operation

10:30-

Culture at 37 degrees Celsius


20:00-

Mini Prep.


20:40-

Digestion Test


20:56-

At 37 degrees Celsius


21:45-

Gel electro...(135 V, 25 min)


PCR

Yesterday's operation is here.

11:15-

Gel electro...(135 V, 27 min)


12:10

Took a picture

Cloning

Digestion

Yesterday's operation is here.

12:37-

Gel electro...(100 V, 40 min)


13:20

into ethyl bromide


13:35

Took a picture and cut the gel.

Then we added 2 mL of ADB Buffer and kept it at 37 degrees Celsius.

DNA Clean & Concentrator

We eluted it 50 uL of dH2O.

SAP処理

  • まぜ表


15:45-

インキュベート it at 37 degrees Celsius.


---30 min---


16:20

We transferred it at 65 degrees Celsius and 失活させた


---15 min---


DNA Clean & Concentrator

We 溶出ed 20 μL.


Gel electro...

17:32

Electro...


18:15

Took a picture


Ligation

18:30

@Room Temperture


21:30

Transformation


21:50

@37 degrees Celsius


23:10

Plating

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