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Team:SDU-Denmark/Notebook
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Project management notebook
Week 1 - BioBricks, primers, protocols and more
Plasmid backbone
7.july: Helle and Mike
We found a plasmid backbone, that works in both e. coli and b. subtilis (that is, both in gram negative and gram positive bacteria). It has a quality star.
The plasmid backbone is called BBa_I742123, and can be found here: http://partsregistry.org/wiki/index.php/Part:BBa_I742123
It seems that the backbone works with all e. coli type, both we're not a 100%.
The backbone is resistant to the following antibiotics: chloramphenicol, neomycin & gentamicin.
Ordering: we've written HQ, requesting the part since we cant figuar out how to get it via the registry.
Inducible promoter
7.July Anna
I found the inducible promoter BBa_R0011 that is regulated by LacI.
It is a strong promoter, that will be on in stains without LacI.
E. coli strain
The plasmid seems to be working in most types (no need for ccdB) Jacob suggests E. coli KG22, which can turns on the operon by adding IPTG.
