Team:Chiba/Project/for painting

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To draw more better picture

  • we screened the "agar media condition" (the thickness & the agar conc.) to make difference in the delay of the LuxR mutant more bigger.


  • Finding more useful condition of medium to demonstrate



We researched the best condition of medium to display our demonstration more clearly.

The protocol of this experiment is here.


  • About the difference of responce of each reporters

First, we did noted above experiment using GFPuv as reporter. Also we did similar experiment using sfGFP, mRFP, mCherry, and mOrange to judge our mutant LuxR.


Therefore, we felt sure that その結果、GFPuvをReporterとして使った場合が最もmutant LuxR間の光り始める時刻の差をはっきりと確認できた。



  • Discussion

The best condition that 最もmutant LuxR間の光り始める時刻の差を確認しやすかった条件は、以下の通りであった。


Reporter : GFPuv

medium : agar 5%

thickness : 7 mm


We thought it could be この条件でデモをやれば、各mutant LuxRの個性（遅れ方の特徴）を最大限に活かすことができると考えた。

そうするとメリハリのあるきれいなアニメーションを描くことが出来るのではないか。


また、使ったReporterによって光り始める時刻に差があったのでこちらにまとめます。

Distinction of fluorescence protein expression between reporters

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