Team:Chiba/Project/reporter
From 2009.igem.org
Time delay depending on the reporters
In our expetriments, the more better condition of medium for the animation demonstration was exxamined with 5 different reporters.
Reporter | GFPuv | mRFP | sfGFP | mCherry | mOrange | |
Time of Recognizing the fastest LuxR [h] | 2 | 3.5 | 4 | 5 | 11 | |
Time of Recognizing the last LuxR [h] | 3 | 24.5 | 50 | 25.5 | 71 |
- Condition
Medium :agar 5%, Thickness 7.0 mm
Temperature : r.t. (ca. 25 degree)
- The plate shown in each photos were obtained at 0, 2, 3.5, 4, 5 h and several days after the incubation (from upper left to bottom right).