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Team:Chiba/Project/for painting
From 2009.igem.org
To draw more better picture
- we screened the "agar media condition" (the thickness & the agar conc.) to enlarge difference in the delay of the LuxR mutant.
- Finding more useful condition of medium to demonstrate
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We researched the best condition of medium to display our demonstration more clearly.
The protocol of this experiment is here.
- About the difference of responce of each reporters
First, we did noted above experiment using GFPuv as reporter. Also we did similar experiment using sfGFP, mRFP, mCherry, and mOrange to judge our mutant LuxR.
As a result, we found that GFPuv is better reporter for our demonstration.
この部分はdiscussionじゃないので消してください[toyota]---------------
- Discussion
The best condition that 最もmutant LuxR間の光り始める時刻の差を確認しやすかった条件は、以下の通りであった。
Reporter : GFPuv
medium : agar 5%
thickness : 7 mm
We thought it could be この条件でデモをやれば、各mutant LuxRの個性（遅れ方の特徴）を最大限に活かすことができると考えた。
そうするとメリハリのあるきれいなアニメーションを描くことが出来るのではないか。
また、使ったReporterによって光り始める時刻に差があったのでこちらにまとめます。
The protocol of this experiment is Distinction of fluorescence protein expression between reporters
