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Team:IIT Madras/Results
From 2009.igem.org
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Results
Comparing the differences in the growth rates of cells with and without plasmids in various media
Growth curves
Fig 8.1: a) DH5a grown in LB containing no antibiotic, LB containing Ampicillin, LB containing Chloramphenicol, LB containing both antibiotics. This clearly shows that DH5a cannot grow in medium with antibiotics. b) RFP (pSB1C3) contanining cells grown in LB containing no antibiotic, LB containing Ampicillin, LB containing Chloramphenicol, LB containing both antibiotics. c) CFP (pSB1A2) contanining cells grown in LB containing no antibiotic, LB containing Ampicillin, LB containing Chloramphenicol, LB containing both antibiotics. d) RFP (pSB1C3) - CFP (pSB1A2) contanining cells grown in LB containing no antibiotic, LB containing Ampicillin, LB containing Chloramphenicol, LB containing both antibiotics.
Fig 8.2: All 4 strains in media without antibiotics. This graph clearly elucidates the fact that the cells which bear a plasmid tend to grow slower than those without it. The DH5a cells show considerably faster growth rate in the exponential phase compared to all the transformed cells. We wish to use this result along with the fact that cells lose plasmids in the absence of an selection pressure to retain it, to direct plasmid loss in a very specific manner, which is the key requirement to achieve a locking system.
Fig 8.3: All 4 strains in their corresponding antibiotic media - DH5a in Lb without antibiotics, RFP (pSB1C3) in LB with Chloramphenicol, CFP (pSB1A2) in LB with Ampicillin, RFP (pSB1C3) - CFP (pSB1A2) cells in LB with Chloramphenicol and Ampicillin.
Fig 8.4: log (OD600) vs Time plot for all 4 strains in media without antibiotics.
Fig 8.5: log (OD600) vs Time plot for all 4 strains in their corresponding antibiotic media - DH5a in Lb without antibiotics, RFP (pSB1C3) in LB with Chloramphenicol, CFP (pSB1A2) in LB with Ampicillin, RFP (pSB1C3) - CFP (pSB1A2) cells in LB with Chloramphenicol and Ampicillin.
Modeling
For updated results, keep checking here.
Fluorescent Imaging
Fig 9: Co-transformed cells with both CFP(1A2) and RFP(1C3)seen under bright field, red filter and cyan filter.
For updated results, keep checking here.
