"
Team:UC Davis/assay for measuring protein localization and activity
From 2009.igem.org
Assay for
measuring protein localization and activity:
We are using western blotting as our assay to measure protein localization and activity, where we have tagged all of our constructs with a 6xHIS tag. This allows us to compare basal GFP or Luciferase activity inside (basal) and outside of the cell (medium).
We are using western blotting as our assay to measure protein localization and activity, where we have tagged all of our constructs with a 6xHIS tag. This allows us to compare basal GFP or Luciferase activity inside (basal) and outside of the cell (medium).
We
may also use fluorescence as an assay by comparing the
brightness of the cell when it keeps it inside the cell, and when it
secretes it into the medium. Both of our test genes, GFP and
Luciferase, are fluorescence proteins.
