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| Oligo Name |
Ordered by... |
Length |
Sequence |
Description |
|
|
| Pimer forward CAT |
Sigma |
64 |
GATCGCAATTGACCAACAAGGAGAAATCTAGATGGCCGGCGAGAAAAAAATCACTGGATATACC |
designed to get CAT-gene + Freigem restriction sites out
of the lab vector no 258 |
|
|
| Primer reverse CAT |
Sigma |
58 |
GTGGCAGGGCGGGGCGACCGGTTAATACTAGTAGCGGCCGCCTGCAGAAGCTTGGGTA |
designed to get CAT-gene + Freigem restriction sites out
of the lab vector no 258 |
|
|
| Short Linker |
Sigma |
18 |
CTAGAGGTGGTTCTGGTA |
GlySerGlyGly |
|
|
| Middle Linker |
Sigma |
30 |
CTAGAGGTGGTTCTGGTACTAGAGGTGGTTCTGGTA |
GlySerGlyGlyx2 |
|
|
| Long Linker |
Sigma |
42 |
CTAGAGGTGGTTCTGGTACTAGAGGTGGTTCTGGTACTAGAGGTGGTTCTGGTA |
GlySerGlyGlyx3 |
|
|
| Primer forward GenIII |
Sigma |
52 |
GAATTCGCGGCCGCTTCTAGATGGCCGGCGATTTTGATTATGAAAAGATGGC |
Insert of
GenIII(restriction sites RFC25) into M13DNA , |
|
|
| Primer reverse GenIII |
Sigma |
53 |
CTGCAGGCGGCCGCTACTAGTATTAACCGGTAGACTCCTTATTACGCAGTATG |
Insert of
GenIII(restriction sites RFC25) into M13DNA , |
|
|
| A4 AGO |
Sigma |
21 |
AAGTTTTTTGGGGTCGAGGTG |
guide oligo complentary to target sequenz, the cutting event will be done after the tenth base 5'3' |
|
|
| A1 AGO |
Sigma |
21 |
ACAACCATCGCCCACGCATAA |
guide oligo complentary to target sequenz, the cutting event will be done after the tenth base 5'3'.The M13ssDNA will
be cut at position 3100 |
|
|
| A2 AGO |
Sigma |
21 |
GGTTTTACTCTGATTCTCTTC |
guide oligo complentary to target sequenz, the cutting event will be done after the tenth base of the oligo 5'3'.The
M13ssDNA will be cut at position 550. |
|
|
| A3 AGO |
Sigma |
21 |
TACCTTCGGGTACTCTTCTAA |
guide oligo complentary to target sequenz, the cutting event will be done after the tenth base 5'3'.The M13ssDNA will
be cut at position 1280. |
|
|
| A5_Ago_pETfragment |
Sigma |
21 |
AAGTTTTTTGGGGTCGAGGTG |
guide
oligo complentary to target sequenz,
the cutting event will be done
after the tenth base 5'3'.The M13ssDNA will be cut at position 100 |
|
|
| Aa BB Primer Middle a |
Sigma |
50 |
TCGTTCATTTTGAAATCCCCTGAACTCTTCTTCACCCACTCTTTTCAGTT |
deleted EcoRI site |
|
|
| Aa BB Primer Middle b |
Sigma |
50 |
GGAAGGTGATATTATGTACTGGCTTTAATAATACTAGTAGCGGCCGCTGCAG |
deleted EcoRI site |
|
|
| Aa BB Primer Start |
Sigma |
45 |
GGTTTAAAAGAGCTTCCTTTCCCATCTAGAAGCGGCCGCGAATTC |
Addon Tail to
add Biobrick prefix |
|
|
| Aa BB Primer Back |
Sigma |
52 |
AACTGAAAAGAGTGGGTGAAGAAGAGTTCAGGGGATTTCAAAATGAACGA |
Addon Tail to
add Biobrick suffix |
|
|
| AGO-target _M13 |
Sigma |
60 |
CTGCAAGCCTCAGCGACCGAATATATCGGTTATGCGTGGGCGATGGTTGTTGTCATTGTC |
5'biotinylated
olgio for phage display |
|
|
| AGO-guide-A1 5' Phos |
Sigma |
21 |
[Phos]ACAACCATCGCCCACGCATAA |
phosporilised(5')
for phage display assay with AGO protein |
|
|
| AGO-guide-A4 5' Phos |
Sigma |
21 |
[Phos]AAGTTTTTTGGGGTCGAGGTG |
phosporilised(5')
for phage display assay with AGO protein |
|
|
| Fok control 1 |
Sigma |
40 |
TTCTACTAATAGTAGTAGCATTAACATCCAATAAATCATA |
1669bp for the bond of gel elctrophoresis , the oligo
hybridisized to the ssDNA enable Fok to cut |
|
|
| Fok control 2 |
Sigma |
40 |
GTCATTTTTGCGGATGGCTTAGAGCTTAATTGCTGAATAT |
1914bp for the bond of gel elctrophoresis, the oligo
hybridisized to the ssDNA enable Fok to cut |
|
|
| Fok control 3 |
Sigma |
40 |
TGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGT |
786bp for the bond of gel elctrophoresis,the oligo
hybridisized to the ssDNA enable Fok to cut |
|
|
| 5'fluo40bp |
Sigma |
40 |
|
Test nucleotide for the in vivo assay |
|
|
| xbaI_RBS-Strep-Fok_a |
Sigma |
40 |
GCTCTAGAGAAGGAGATATACTATGGCCGGCTGGAGCCAT |
Insert a RBS befor the StrepTag |
|
|
| StrepFoka_r |
Sigma |
28 |
GAAGCTTCTGCAGCGGCCGCTACTAGTA |
Insert a RBS befor the StrepTag |
|
|
| XbaI_DsbAss_AgeI_2r |
Sigma |
62 |
CCGGTCGCCGATGCGCTAAACGCTAAAACTAAACCAGCCAGCGCCAGCCAAATCTTTTTCAT |
olgio
with xbaI and ageI ends , can linked on DsbA signalsequenz |
|
|
| pBAD-TOPO f |
Sigma |
34 |
GTCCCCCCGGGAACCCCGCTTATTAAAAGCATTC |
add-on tail primer for pcr to remove the two AgeI sites of
the vector and to introduce the bb-pre and suffix |
|
|
| pBAD-TOPO r |
Sigma |
56 |
TCCCCCCGGGCTGCAGTATGAATTCACTCCTTCTTAAAGTTAAACAAAATTATTTC |
add-on tail primer for pcr to remove the two AgeI sites of
the vector and to introduce the bb-pre and suffix |
|
|
| XbaI_NgoMIV_DsbA forward |
Sigma |
19 |
CGCTTCTAGATGGCCGGCA |
add-on tail primer to generte a FOS-DsbA-Split-Fok_a part |
|
|
| DsbA-Fos |
Sigma |
36 |
CGTTTAGCGCATCGGCGCACCATCACCACCACCATG |
add-on tail primer to generte a FOS-DsbA-Split-Fok_a part |
|
|
| Fos-SplitFok_a |
Sigma |
38 |
GGAGTTCATCCTGGCAGCACGACCAGCCTGTAAGATTC |
add-on tail primer to generte a FOS-DsbA-Split-Fok_a part |
|
|
| SplitFok_a age_Spe_not_pst |
Sigma |
17 |
AGCTCTGCAGCGGCCGC |
add-on tail primer to generte a FOS-DsbA-Split-Fok_a part |
|
|
| 80mer Fok control |
Sigma |
80 |
AGTTCGGTTCCCTTATGATTGACCGTCTGCGCCTCGTTCCGGCTAAGTAACATGGAGCAGGTCGCGGATTTCGACACAAT |
target oligonucleotide for Fok cleavage assay |
|
|
| 80mer Fok control |
Sigma |
80 |
[Cy3]AGTTCGGTTCCCTTATGATTGACCGTCTGCGCCTCGTTCCGGCTAAGTAACATGGAGCAGGTCGCGGATTTCGACACAAT |
target oligonucleotide for Fok cleavage assay with
fluorescine mark |
|
|
| Standard 35 forward |
Sigma |
25 |
CCGAATTCGCGGCCGCTTCTAGATG |
freiburg stadard primer (standard 25) to amplify insert |
|
|
| Standard 35 reverse |
Sigma |
29 |
GCTCTGCAGCGGCCGCTACTAGTATTAAC |
freiburg stadard primer (standard 25) to amplify insert |
|
|
| pr_fwd_Xba_SDII_TorA |
Sigma |
55 |
ATATAAATTCTAGATAACGAGGGCAAATCATGAACAATAACGATCTCTTTCAGGC |
Shine-Dalgarno sequence II TorA(signal sequence for
periplasm transport) |
|
|
| ssDNA_prod_pET39b+ |
Sigma |
23 |
CGGATCCGATATCGCCATGGTTG |
primer for modifies thermocycler protocoll to produce
ssDNA |
|
|
| fokm_li_xbaI_ageI |
Sigma |
90 |
CTAGATGGCCGGCGGTTCTGGTGGTGGTTCTGGCGGTGGTTCTGGAGGTAGTTCTGGCGGTGGATCTGGAGGCGGTTCTGGGTCAGGATC |
36 GS linker wrong |
|
|
| fok_m_link_NgoMIV_HINFI_compl |
Sigma |
91 |
CCGGCGGTGGTTCTGGTGGTGGTTCTGGCGGTGGTTCTGGAGGTAGTTCTGGCGGTGGATCTGGAGGCGGTTCTGGGTCAGGATCTGGTGATGGTTCTGGCTCTGGG |
36 Gs linker wrong complement |
|
|
| o_diA1_5dig_site1 |
Purimex |
16 |
CGGAACGAGGCGCAGA |
Modified oligonucleotid to guide the Fok-Complex to the
target sequence via digoxigenin |
|
|
| o_diAB1_5fluo_site1 |
Purimex |
16 |
CGGTCAATCATAAGGG |
Modified oligonucleotid to guide the Fok-Complex to the
target sequence via fluorescin |
|
|
| o_diB1_15dig_site1 |
Purimex |
30 |
CCATGTTACTTAGCCGGAACGAGGCGCAGA |
Modified oligonucleotid to guide the Fok-Complex to the
target sequence via digoxigenin |
|
|
| o_mono1_3fluo_14dig_site1 |
Purimex |
30 |
CATGTTACTTAGCCGGAACGAGGCGCAGAC |
Modified oligonucleotid to guide the Fok-Complex to the
target sequence via digoxigenin and fluorecine |
|
|
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