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Minnesota-experimental/1 July 2009
From 2009.igem.org
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- Flow cytometry data was collected for samples from 6-29. (TTL, mutant at position 4).
- PCR products from 6-30 were cleaned using a QIAquick column protocol.
- TNN, TTN, and TTL promoter constructs mutant at position 5 were ligated into the pGlow-TOPO vector (Invitrogen).
- Ligation products were transformed into TOP10 cells; cells were spread on antibiotic LB agar plates and allowed to grow at 37 °C overnight.
