Team:Virginia/Approach

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Approach.png

The endogenuos E. coli arsenic detoxification pathway

We had two goals:

  1. Remove the arsenite efflux mechanism in E. coli
  2. Create a sequestration mechanism


Escherichia coli uses the [http://ecoliwiki.net/colipedia/index.php/arsB arsB] ion transporter to specifically transport arsenite (As(III)) ions out of the cytoplasm. We obtained a single gene knock-out from the [http://ecoli.naist.jp/gb6/Resources/deletion/deletion.html Keio Collection] for arsB.


Safety

  1. Would any of your project ideas raise safety issues in terms of: (researcher safety, public safety, or environmental safety?)
    • The University's Environmental Health and Safety department was involved in our project from the beginning. Team members absolved training to handle arsenic and proper precautions were taken in the lab for storage.
  2. Is there a local biosafety group, committee, or review board at your institution?
    • See above.
  3. What does your local biosafety group think about your project?
    • See above.
  4. Do any of the new BioBrick parts that you made this year raise any safety issues?
    • Hopefully they will make the world safer rather than raise safety issues! (No)
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