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Team:Illinois/sRNA Library
From 2009.igem.org
sRNA Library Team
Goals: Our team will be characterizing various small RNAs and submitting them to the Parts Registry as working biobricks. We will insert the sRNA gene into a high-copy plasmid and its target sequence before the GFP gene in a low-copy plasmid. We will then transform both plasmids into E. coli cells. The sRNA should successfully repress expression of the GFP gene and result in a decrease in fluorescence. We will be using a protocol documented in a paper by Urban and Vogel (please see the Protocols page).
Click on a link below to view information pertaining to a specific sRNA, including gene sequences and primers used.
