Team:Illinois/Hybrid Promoter

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Hybrid Promoter

Goals: The goal of this side-project is the create a hybrid or combinatorial promoter that accepts two inputs. This will be used in the creation of an AND logic gate.

Papers of Interest:

[http://www.nature.com/nature/journal/v420/n6912/full/nature01257.html Engineered gene circuits] Jeff Hasty, David McMillen & J. J. Collins

[http://aem.asm.org/cgi/content/abstract/75/3/637 Construction and Enhancement of a Minimal Genetic AND Logic Gate] Daniel J. Sayut, Yan Niu, and Lianhong Sun

[http://www.sciencemag.org/cgi/content/full/296/5572/1466 Combinatorial Synthesis of Genetic Networks] Cabrevelin C. Guet, Michael B. Elowitz, Weihong Hsing, Stanislas Leibler

[http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2132448 Programming gene expression with combinatorial promoters] Robert Sidney Cox, III, Michael G Surette, and Michael B Elowitz

Biobricks of Interest:

BBa_k117004 pLacI-GFP. induction of IPTG = gfp expression BBa_J58101 promoter activated synergistically by cl and CRP with the reporter RFP

BBa_l7100 has TetR repressible promoter and GFP generator produces fluorescence in either absence of TetR or presence of aTc

BBa_R0010 lacl regulated promoter BBa_10500 inducible pBad/araC promoter

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