Agarose Gels
From 2009.igem.org
Agarose Gel
- 50mL 0.5x TBE buffer
- Agarose (To determine number of grams to use, multiply volume (50mL) by percentage of gel. For example, use 1.5g of agarose in a 3% agarose gel.)
- 2.5μL ethidium bromide
When making a small gel use 50mL of TBE buffer. The dispenser is on the bench behind ours in a large Nalgene container. Use the graduated cylinder next to it, but only for the TBE buffer.