From 2009.igem.org
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Spun tubes #1-8 for 3 minutes
Combined tubes #1-4 and tubes #5-8
Added 300x TENS
Mix by inversion
Added 150x sodium acetate
Mix by inversion
Spun for 2.5 minutes
Transfered Sup to clean tube
Added 1 mL of EtOH (ethanol)
Spin for 5 minutes
Washed pellet in 70% EtOH 300x
Spin for 5 minutes
Resuspend 30x TE
Digest w/ EcoRI
5x DNA
2x Buffer
11x dH2O
1x EcoRI
Incubate at 37C at 11:45AM
Saw no bands
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