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Team:KULeuven/14 July 2009
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Contents |
Progress
To do:
- Strain selection
- Odor-free E.coli available on partsregistry () [ordered]
- Order iobricks for Vanillin detection [Done]
- Primer selection for the promotor [Done]
- Send mail to Institut for Biologie-Mikrobiologie ([http://www.ncbi.nlm.nih.gov/pubmed/19240136 The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli]) [Done]
- Biobricks for vanillin synthesis
- Send mail to University of Tuscia [Done]
- Order:
- (sam8 (tyrosine-ammonia lyase) coding sequence) [on hold]
- (sam5 (coumarate hydroxylase) coding sequence) [on hold]
- (COMT gene with ribosome binding site) [Available in kit]
Extra info on Vanillin synthesis
http://www.ncbi.nlm.nih.gov/pubmed/16232583 1 Construction of recombinants Pseudomonas putida BO14 and Escherichia coli QEFCA8 for ferulic acid biotransformation to vanillin, B C Okeke and V Venturi ,J Biosci Bioeng. 1999; 88(1): 103–106.
Concept
