"
Team:KULeuven/14 July 2009
From 2009.igem.org
Contents |
Progress
To do:
- Strain selection
- Odor-free E.coli available on partsregistry () [ordered]
- Order iobricks for Vanillin detection [Done]
- Primer selection for the promotor [Done]
- Send mail to Institut for Biologie-Mikrobiologie ([http://www.ncbi.nlm.nih.gov/pubmed/19240136 The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli]) [Done]
- Biobricks for vanillin synthesis
- Send mail to University of Tuscia [Done]
- Order:
- (sam8 (tyrosine-ammonia lyase) coding sequence) [on hold]
- (sam5 (coumarate hydroxylase) coding sequence) [on hold]
- (COMT gene with ribosome binding site) [Available in kit]
Extra info on Vanillin synthesis
http://www.ncbi.nlm.nih.gov/pubmed/16232583 1 Construction of recombinants Pseudomonas putida BO14 and Escherichia coli QEFCA8 for ferulic acid biotransformation to vanillin, B C Okeke and V Venturi ,J Biosci Bioeng. 1999; 88(1): 103–106.
http://www.ncbi.nlm.nih.gov/pubmed/14602615 2Highly Efficient Biotransformation of Eugenol to Ferulic Acid and Further Conversion to Vanillin in Recombinant Strains of Escherichia coli, Jörg Overhage, Alexander Steinbüchel, and Horst Priefert, Appl Environ Microbiol. 2003 November; 69(11): 6569–6576. doi: 10.1128/AEM.69.11.6569-6576.2003.
http://www.ncbi.nlm.nih.gov/pubmed/10616715 3Biotransformation of eugenol to vanillin by a mutant of Pseudomonas sp. strain HR199 constructed by disruption of the vanillin dehydrogenase (vdh) gene, J Overhage, H Priefert, J Rabenhorst, and A Steinbüchel, Appl Microbiol Biotechnol. 1999 November; 52(6): 820–828.
Concept
