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Team:KULeuven/Abstract
From 2009.igem.org
Essencia Coli
The main idea is to synthesize a molecule 'X' and at the same time measure the concentration of this molecule outside the cell in order to keep the concentration constant. This concentration can then be modulated using light intensity. This creates a controll system with feedback from the concentration.
For proof of concept the molecule 'X' is set to be vanillin. The light receptor will synthesize molecule B on irradiation of bleu light.Vanillin will be synthesized and can freely diffuse out of the cell. The concentration outside the cell is measured with a vanillin receptor.
how we'll control the concentration
Biobricks
all the parts we are planning on using/making:
the ribokey controlled by the blue light receptor
Vanillinsynthesis with ribolock in front.
Vanillin receptor.
Antikey that is produced when the vanillin concentration reaches high levels.
to be constructed
