"
Team:KULeuven/Modelling
From 2009.igem.org
Contents |
Blue Light Sensor
Overview
The purpose of the sensor is to increase the production of the RIBOKEY under the control of the intensity of the incident blue light.
Input: Blue light (<math>\lambda = 470</math>)
Output: mRNA_RIBOKEY
The biology works as follows, YcgF protein is believed to dimerize upon photo-excitation. The YcgE protein is a repressor for the transcription of the mRNA_RIBOKEY, only the dimer form of YcgF is capable of interacting with the operator-bound YcgE and to release it from the DNA.
The entire network of interactions is visualized in the diagram below
Reactions
Dimerization of YcgF
"Transient Dimerization and Conformational Change of a BLUF Protein: YcgF", [http://pubs.acs.org/doi/full/10.1021/ja065682q]
YcgF - YcgE interaction
Transcription of the RIBOKEY
Degradation of the RIBOKEY
References
[1] Natalia Tschowri, Susan Busse and Regine Hengge, "The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli"
