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Team:KULeuven/Modelling
From 2009.igem.org
Contents |
Blue Light Sensor
Overview
The purpose of the sensor is to increase the production of the RIBOKEY under the control of the intensity of the incident blue light.
Input: Blue light (<math>\lambda = 470</math>)
Output: mRNA_RIBOKEY
The biology works as follows, YcgF protein is believed to dimerize upon photo-excitation. The YcgE protein is a repressor for the transcription of the mRNA_RIBOKEY, only the dimer form of YcgF is capable of interacting with the operator-bound YcgE and to release it from the DNA.
The entire network of interactions is visualized in the diagram below
Reactions
Dimerization of YcgF
"Transient Dimerization and Conformational Change of a BLUF Protein: YcgF", [http://pubs.acs.org/doi/full/10.1021/ja065682q]
YcgF - YcgE interaction
"The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli" [http://genesdev.cshlp.org/content/23/4/522.full.pdf+html]
Transcription of the RIBOKEY
Degradation of the RIBOKEY
"Light Induced Structural Changes of a Full-length Protein and Its BLUF Domain in YcgF(Blrp), a Blue-Light Sensing Protein That Uses FAD (BLUF)" [http://pubs.acs.org/doi/abs/10.1021/bi051820x]
References
[1] Natalia Tschowri, Susan Busse and Regine Hengge, "The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli"
