Virginia Commonwealth/16 July 2009

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Revision as of 14:31, 16 July 2009 by Trentay (Talk | contribs)
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Contents

Saturday 06 June 2009

Results

  • Results of Retransformation with pSB4C5 backbone in NEB 10 beta two days after transformation
Promoter Reporter Resistance/Plate Growth Observation
J23101 E0240 Cm no growth
J23101 J06702 Cm 21 colonies
J23102 E0240 Cm 3 colonies
J23102 J06702 Cm 8 colonies
J23103 E0240 Cm 2 colonies
J23103 J06702 Cm 23 colonies
J23104 E0240 Cm 9 colonies
J23104 J06702 Cm 12 colonies
J23105 E0240 Cm no growth
J23105 J06702 Cm 14 colonies
J23107 E0240 Cm 3 colonies
J23107 J06702 Cm 3 colonies
  • No growth was seen in 5mL culture taken from NEB 10 beta cell freezer stock
    • Vial may have thawed out too much after cells were taken out for the electrocompetency procedure

Trentay 14:31, 16 July 2009 (UTC)


Tasks

Maria and Afton

  • Digest: E0240, pSB4C5, 110, 113, 116,

Wetlab

Maria and Afton