Team:KULeuven/Modelling

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Contents

Blue Light Sensor

Overview

The purpose of the sensor is to increase the production of the RIBOKEY under the control of the intensity of the incident blue light.

Input: Blue light (<math>\lambda = 470</math>)
Output: mRNA_RIBOKEY

Sensor interface

The biology works as follows, YcgF protein is believed to dimerize upon photo-excitation. The YcgE protein is a repressor for the transcription of the mRNA_RIBOKEY, only the dimer form of YcgF is capable of interacting with the operator-bound YcgE and to release it from the DNA.

The entire network of interactions is visualized in the diagram below

Regulation of the production of mRNA key by incident blue light

Reactions

Dimerization of YcgF

"Transient Dimerization and Conformational Change of a BLUF Protein: YcgF", [http://pubs.acs.org/doi/full/10.1021/ja065682q]

Sensor interface

"Light Induced Structural Changes of a Full-length Protein and Its BLUF Domain in YcgF(Blrp), a Blue-Light Sensing Protein That Uses FAD (BLUF)" [http://pubs.acs.org/doi/abs/10.1021/bi051820x]

YcgF - YcgE interaction

"The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli" [http://genesdev.cshlp.org/content/23/4/522.full.pdf+html]

Transcription of the RIBOKEY

https://2008.igem.org/Team:KULeuven/Model/Filter

Degradation of the RIBOKEY

https://2008.igem.org/Team:KULeuven/Model/Filter

References

[1] Natalia Tschowri, Susan Busse and Regine Hengge, "The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli"