Virginia Commonwealth/15 July 2009

From 2009.igem.org

Revision as of 18:08, 17 July 2009 by Albergca (Talk | contribs)

Contents

Saturday 06 June 2009

Results

  • Results of Retransformation with pSB4C5 backbone in NEB 10 beta
Promoter Reporter Resistance/Plate Growth Observation
J23101 E0240 Cm
J23101 J06702 Cm
J23102 E0240 Cm
J23102 J06702 Cm
J23103 E0240 Cm
J23103 J06702 Cm
J23104 E0240 Cm
J23104 J06702 Cm
J23105 E0240 Cm
J23105 J06702 Cm
J23107 E0240 Cm
J23107 J06702 Cm
  • Controls with NEB 10 beta
Control Resistance Plate Growth Observation
Positive (+) none LB lawn
Electroshocked (+) none LB lawn
Negative (-) none Cm no cell growth

Trentay 16:59, 15 July 2009 (UTC)

Craig and Clay

  • The ampicillin plates again showed no growth. However, the non-selective plates displayed lawn growth.

Tasks

Maria and Afton

  • Make more electrocompetent NEB 10 beta
    • 500 mL LB autoclaved in 1 L flask
    • 1 L LB autoclaved
    • 2mL of NEB 10 beta overnight culture was diluted in 4mL of LB broth
  • Two bottles of LB Agar were prepared
    • for Amp and Cm plates
  • Three bottles of LB prepared
    • two liquid LB+Amp will be prepared
    • one liquid LB+Cm will be prepared
  • Let transformed BioBrick parts continue to grow overnight (on plates) before colonies are picked
  • Make cell stock of Biobrick part: J23106, J06702, pSB4C5
    • 2mL of BioBrick overnight culture was diluted in 4mL of LB broth
  • Organize Notebook(s)

Trentay 16:59, 15 July 2009 (UTC)

Craig and Clay

  • The cells growing on the non-selective media will be streaked onto plates containing kanamycin and plates containing chloramphenicol.

Wetlab

Maria and Afton

  • All LB was prepared and autoclaved
  • Overnight culture dilutions were completed
  • Electrocompetency of NEB 10 beta was completed
  • LB and Sterile water was made
  • 10% glycerol stock was made

Trentay 14:13, 16 July 2009 (UTC)

Craig and Clay

  • Cells possibly containing the limonene synthesis related parts grown on the plates containing no antibiotics were taken and plated onto kanamycin-selective and chloramphenicol-selective plates.