Biobrick/
Ligation Name |
Description |
Creation Date |
N° usage |
Strain |
Vector |
Quality Control |
| J23101(J101) |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
| J23102(J102) |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
| J23103(J103) |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
| J23104(J104) |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
| J23105(J105) |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
| J23106(J106) |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
| J23110(J110) |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
| J23112(J112) |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
| J23113(J113) |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
| J23114(J114) |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
| J23116(J116) |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
| J23117(J117) |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
| J23118(J118) |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
| E0240(GFP3) |
GFP protein generator
(RBS-GFP-TT) |
May 25 |
6 |
TOP10 |
pSB1A2 |
length ok (PCR) sequence ok (MIT) |
| K131009(E2) |
colE2 operon:ceaB-ceiB-Tweak-celB-T (with RBS) |
May 28 |
1 |
TOP10 |
pSB1AK3 |
sequence BAD in prefix,suffix,celB (MIT,BMR) |
| K117000(celB) |
colE7 lysis gene |
May 28 |
1 |
TOP10 |
pSB1A2 |
sequence ok (MIT) |
| K124017(lysis21) |
Phage Lysis Cassette |
May 28 |
0 |
TOP10 |
pSB1AK3 |
sequence BAD (MIT) |
| B0015(TT) |
Double terminator |
May 28 |
2 |
TOP10 |
pSB1AK3 |
sequence ok (MIT) |
| I732017 (RBS-lacZ) |
RBS-lacZ |
May 28 |
1 |
TOP10 |
pSB1A2 |
long part (MIT) |
| B0030 |
RBS 0.6 |
Jun 4 |
1 |
TOP10 |
pSB1A2 |
sequence ok (MIT,BMR) |
| B0031 |
RBS 0.07 |
Jun 4 |
0 |
TOP10 |
pSB1A2 |
sequence ok (MIT) |
| B0032 |
RBS 0.3 |
Jun 4 |
0 |
TOP10 |
pSB1A2 |
sequence ok (MIT) |
| B0033 |
RBS 0.01 |
Jun 4 |
0 |
TOP10 |
pSB1A2 |
sequence ok (MIT) |
| B0034 |
RBS 1 |
Jun 4 |
0 |
TOP10 |
pSB1A2 |
sequence ok (MIT) |
| P0440(P04) |
RBS-tetR-TT |
Jun 4 |
1 |
TOP10 |
pSB1A2 |
length ok (PCR) sequence ok (MIT) |
| R0040(Ptet) |
Ptet |
Jun 4 |
1 |
TOP10 |
pSB1A2 |
sequence ok (MIT) |
| T9002 |
Ptet-RBS-luxR-TT-Plux-RBS-GFP-TT |
Jun 4 |
3 |
TOP10 |
pSB1A3 |
sequence BAD (MIT) - sequence ok (BMR) |
| J23100(J100bis) |
constitutive promoter |
Jun 4 |
3 |
TOP10 |
J61002(Amp) |
sequence ok (MIT,BMR) |
| J23101(J101bis) |
constitutive promoter |
Jun 4 |
2 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
| J23118(J118bis) |
constitutive promoter |
Jun 4 |
3 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
| A1 |
J23100(Jun 4)-E0240
(Pcon-RBS-GFP-TT) |
Jun 11 |
3 |
TOP10 |
pSB1A2 |
length ok (PCR) |
| A2 |
J23101(Jun 4)-E0240
(Pcon-RBS-GFP-TT) |
Jun 11 |
1 |
TOP10 |
pSB1A2 |
length ok (PCR) |
| A3-1 |
I732017-B0015
(RBS-lacZ-TT) |
Jun 12 |
2 |
TOP10 |
pSB1AK3 |
length ok (digestion) sequence ok -long part- (BMR) |
| A4 |
P0440-R0040
(RBS-tetR-TT-Ptet) |
Jun 11 |
2 |
TOP10 |
pSB1A2 |
length ok (PCR) sequence ok (BMR) |
| A5 |
A4-A3
(RBS-tetR-TT-Ptet-RBS-lacZ-TT) |
Jun 19 |
0 |
TOP10 |
pSB1AK3 |
sequence ok -long part- (BMR) |
| A6 |
J23100(Jun 4)-A4
(Pcon-RBS-tetR-TT-Ptet) |
Jun 19 |
1 |
TOP10 |
pSB1A2 |
length ok (PCR) sequence ok (BMR) |
| BOL1 |
J23118-B0034-C0012-B0015
(Pcon-RBS-lacI-TT) |
Jun 23 |
1 |
TOP10 |
pSB1A2 |
sequence ok (BMR) |
| A7 |
J23118(Jun 4)-E0240
(Pcon-RBS-GFP-TT) |
Jun 25 |
1 |
TOP10 |
pSB1A2 |
length ok (PCR) |
| R0011(Plac) |
Plac |
Jun 25 |
0 |
TOP10 |
pSB1A2 |
sequence ok (MIT) |
| K112808(ENTERO) |
holin-lysozyme-T-Pconweak-antiholin-T (with RBSs) |
Jun 25 |
0 |
TOP10 |
pSB1A2 |
sequence ok -long part- (MIT) |
| A8 |
A4-E0240
(RBS-tetR-TT-Ptet-RBS-GFP-TT) |
Jun 29 |
2 |
TOP10 |
pSB1A2 |
NON PURE PLASMID! (digestion) but works |
| A9 |
A6-E0240
(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT) |
Jun 29 |
2 |
TOP10 |
pSB1A2 |
NON PURE PLASMID! (digestion) but works |
A10
thrown away |
K117000-B0015
(celB-TT) |
Jun 30 |
2 |
TOP10 |
pSB1AK3 |
sequence BAD - repeat ligation |
| A8pg |
A4-E0240
(RBS-tetR-TT-Ptet-RBS-GFP-TT) |
Jul 3 |
0 |
TOP10 |
pSB1A2 |
length ok (digestion) |
| A9pg |
A6-E0240
(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT) |
Jul 3 |
2 |
TOP10 |
pSB1A2 |
length ok (digestion) |
| A11-1 |
BOL1-R0011
(Pcon-RBS-lacI-TT-Plac) |
Jul 10 |
1 |
TOP10 |
pSB1A2 |
length ok (PCR) |
| A11-3 |
BOL1-R0011
(Pcon-RBS-lacI-TT-Plac) |
Jul 10 |
0 |
TOP10 |
pSB1A2 |
length ok (PCR) |
| A11-6 |
BOL1-R0011
(Pcon-RBS-lacI-TT-Plac) |
Jul 10 |
0 |
TOP10 |
pSB1A2 |
length ok (PCR) |
| F2620 |
Ptet-RBS-luxR-TT-Plux
|
Jul 10 |
1 |
TOP10 |
pSB1A2 |
sequence BAD (MIT) |
| A12-2 |
J23118(Jun 4)-A4
|
Jul 16 |
0 |
TOP10 |
pSB1A2 |
length ok (PCR) |
| A12-3 |
Ptet-RBS-luxR-TT-Plux
|
Jul 16 |
0 |
TOP10 |
pSB1A2 |
length ok (PCR) |
| A14-4 |
A11-E0240
|
Jul 16 |
0 |
TOP10 |
pSB1A2 |
NON PURE PLASMID! (digestion) |
| MRGENE1 |
pdc
|
Jul 17 |
0 |
TOP10 |
check |
antibiotic ok
sequence ok (Mr Gene) |
| MRGENE1-2 |
pdc
|
Jul 17 |
0 |
TOP10 |
check |
antibiotic ok
sequence ok (Mr Gene) |
| MRGENE1-3 |
pdc
|
Jul 17 |
0 |
TOP10 |
check |
antibiotic ok
sequence ok (Mr Gene) |
| MRGENE2 |
adhB
|
Jul 17 |
0 |
TOP10 |
check |
antibiotic ok
sequence ok (Mr Gene) |
| MRGENE2-2 |
adhB
|
Jul 17 |
0 |
TOP10 |
check |
antibiotic ok
sequence ok (Mr Gene) |
| MRGENE2-3 |
adhB
|
Jul 17 |
0 |
TOP10 |
check |
antibiotic ok
sequence ok (Mr Gene) |
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