| June
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| [http://2009.igem.org/Illinois/1_June_2009 1]
| [http://2009.igem.org/wiki/index.php?title=Illinois/2_June_2009&action=edit 2]
| [http://2009.igem.org/wiki/index.php?title=Illinois/3_June_2009&action=edit 3]
| [http://2009.igem.org/wiki/index.php?title=Illinois/4_June_2009&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Illinois/5_June_2009&action=edit 5]
| [http://2009.igem.org/wiki/index.php?title=Illinois/6_June_2009&action=edit 6]
| [http://2009.igem.org/wiki/index.php?title=Illinois/7_June_2009&action=edit 7]
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| [http://2009.igem.org/wiki/index.php?title=Illinois/8_June_2009&action=edit 8]
| [http://2009.igem.org/wiki/index.php?title=Illinois/9_June_2009&action=edit 9]
| [http://2009.igem.org/wiki/index.php?title=Illinois/10_June_2009&action=edit 10]
| [http://2009.igem.org/wiki/index.php?title=Illinois/11_June_2009&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Illinois/12_June_2009&action=edit 12]
| [http://2009.igem.org/wiki/index.php?title=Illinois/13_June_2009&action=edit 13]
| [http://2009.igem.org/wiki/index.php?title=Illinois/14_June_2009&action=edit 14]
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| [http://2009.igem.org/Illinois/15_June_2009 15]
| [http://2009.igem.org/Illinois/16_June_2009 16]
| [http://2009.igem.org/Illinois/17_June_2009 17]
| [http://2009.igem.org/Illinois/18_June_2009 18]
| [http://2009.igem.org/Illinois/19_June_2009 19]
| [http://2009.igem.org/wiki/index.php?title=Illinois/20_June_2009&action=edit 20]
| [http://2009.igem.org/wiki/index.php?title=Illinois/21_June_2009&action=edit 21]
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| [http://2009.igem.org/wiki/index.php?title=Illinois/22_June_2009&action=edit 22]
| [http://2009.igem.org/Illinois/23_June_2009 23]
| [http://2009.igem.org/Illinois/24_June_2009 24]
| [http://2009.igem.org/Illinois/25_June_2009 25]
| [http://2009.igem.org/Illinois/26_June_2009 26]
| [http://2009.igem.org/wiki/index.php?title=Illinois/27_June_2009&action=edit 27]
| [http://2009.igem.org/wiki/index.php?title=Illinois/28_June_2009&action=edit 28]
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| [http://2009.igem.org/Illinois/29_June_2009 29]
| [http://2009.igem.org/Illinois/30_June_2009 30]
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| July
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| [http://2009.igem.org/Illinois/1_July_2009 1]
| [http://2009.igem.org/Illinois/2_July_2009 2]
| [http://2009.igem.org/Illinois/3_July_2009 3]
| [http://2009.igem.org/wiki/index.php?title=Illinois/4_July_2009&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Illinois/5_July_2009&action=edit 5]
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| [http://2009.igem.org/Illinois/6_July_2009 6]
| [http://2009.igem.org/Illinois/7_July_2009 7]
| [http://2009.igem.org/wiki/index.php?title=Illinois/8_July_2009&action=edit 8]
| [http://2009.igem.org/Illinois/9_July_2009 9]
| [http://2009.igem.org/Illinois/10_July_2009 10]
| [http://2009.igem.org/wiki/index.php?title=Illinois/11_July_2009&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Illinois/12_July_2009&action=edit 12]
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| [http://2009.igem.org/Illinois/13_July_2009 13]
| [http://2009.igem.org/Illinois/14_July_2009 14]
| [http://2009.igem.org/Illinois/15_July_2009 15]
| [http://2009.igem.org/Illinois/16_July_2009 16]
| [http://2009.igem.org/Illinois/17_July_2009 17]
| [http://2009.igem.org/wiki/index.php?title=Illinois/18_July_2009&action=edit 18]
| [http://2009.igem.org/wiki/index.php?title=Illinois/19_July_2009&action=edit 19]
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| [http://2009.igem.org/Illinois/20_July_2009 20]
| [http://2009.igem.org/Illinois/21_July_2009 21]
| [http://2009.igem.org/Illinois/22_July_2009 22]
| [http://2009.igem.org/Illinois/23_July_2009 23]
| [http://2009.igem.org/Illinois/24_July_2009 24]
| [http://2009.igem.org/wiki/index.php?title=Illinois/25_July_2009&action=edit 25]
| [http://2009.igem.org/wiki/index.php?title=Illinois/26_July_2009&action=edit 26]
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| [http://2009.igem.org/wiki/index.php?title=Illinois/27_July_2009&action=edit 27]
| [http://2009.igem.org/wiki/index.php?title=Illinois/28_July_2009&action=edit 28]
| [http://2009.igem.org/wiki/index.php?title=Illinois/29_July_2009&action=edit 29]
| [http://2009.igem.org/wiki/index.php?title=Illinois/30_July_2009&action=edit 30]
| [http://2009.igem.org/wiki/index.php?title=Illinois/31_July_2009&action=edit 31]
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| August
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| [http://2009.igem.org/wiki/index.php?title=Illinois/1_August_2009&action=edit 1]
| [http://2009.igem.org/wiki/index.php?title=Illinois/2_August_2009&action=edit 2]
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| [http://2009.igem.org/wiki/index.php?title=Illinois/3_August_2009&action=edit 3]
| [http://2009.igem.org/wiki/index.php?title=Illinois/4_August_2009&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Illinois/5_August_2009&action=edit 5]
| [http://2009.igem.org/wiki/index.php?title=Illinois/6_August_2009&action=edit 6]
| [http://2009.igem.org/wiki/index.php?title=Illinois/7_August_2009&action=edit 7]
| [http://2009.igem.org/wiki/index.php?title=Illinois/8_August_2009&action=edit 8]
| [http://2009.igem.org/wiki/index.php?title=Illinois/9_August_2009&action=edit 9]
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| [http://2009.igem.org/wiki/index.php?title=Illinois/10_August_2009&action=edit 10]
| [http://2009.igem.org/wiki/index.php?title=Illinois/11_August_2009&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Illinois/12_August_2009&action=edit 12]
| [http://2009.igem.org/wiki/index.php?title=Illinois/13_August_2009&action=edit 13]
| [http://2009.igem.org/wiki/index.php?title=Illinois/14_August_2009&action=edit 14]
| [http://2009.igem.org/wiki/index.php?title=Illinois/15_August_2009&action=edit 15]
| [http://2009.igem.org/wiki/index.php?title=Illinois/16_August_2009&action=edit 16]
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| [http://2009.igem.org/wiki/index.php?title=Illinois/17_August_2009&action=edit 17]
| [http://2009.igem.org/wiki/index.php?title=Illinois/18_August_2009&action=edit 18]
| [http://2009.igem.org/wiki/index.php?title=Illinois/19_August_2009&action=edit 19]
| [http://2009.igem.org/wiki/index.php?title=Illinois/20_August_2009&action=edit 20]
| [http://2009.igem.org/wiki/index.php?title=Illinois/21_August_2009&action=edit 21]
| [http://2009.igem.org/wiki/index.php?title=Illinois/22_August_2009&action=edit 22]
| [http://2009.igem.org/wiki/index.php?title=Illinois/23_August_2009&action=edit 23]
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| [http://2009.igem.org/wiki/index.php?title=Illinois/24_August_2009&action=edit 24]
| [http://2009.igem.org/wiki/index.php?title=Illinois/25_August_2009&action=edit 25]
| [http://2009.igem.org/wiki/index.php?title=Illinois/26_August_2009&action=edit 26]
| [http://2009.igem.org/wiki/index.php?title=Illinois/27_August_2009&action=edit 27]
| [http://2009.igem.org/wiki/index.php?title=Illinois/28_August_2009&action=edit 28]
| [http://2009.igem.org/wiki/index.php?title=Illinois/29_August_2009&action=edit 29]
| [http://2009.igem.org/wiki/index.php?title=Illinois/30_August_2009&action=edit 30]
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| [http://2009.igem.org/wiki/index.php?title=Illinois/31_August_2009&action=edit 31]
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July 13
Hybrid Promoter
Today in lab we inoculated a colony of the cells that we transformed the biobricks into yesterday.
Concerns: We want the sRNA gene on a high copy plasmid and the target sequence on low copy plasmid. This could require up to 7 different plasmids to transform into the cell. We need to look into the feasibility of this.
Other things to consider:
- Can we put all of the sRNA expressing genes and their promoters on one plasmid(with all the target sequences and the genes that go with)? would this be too many ligations? How would we tell if they all worked? Run a gel?
- Would we co-transform the plasmids or do them separately?
Modeling
The transformation of BBa_K113009 yielded colonies, whereas the transformation of BBa_I0500 did not. We will have to redo the transformation of BBa_I0500.
We ran a PCR to amplify the pSB3K3 plasmid backbone for testing our promoters. For some reason, the gel we ran indicated a 6kbp band when it should have been 2.75kbp.
Back to Notebook page
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