| June
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| [http://2009.igem.org/Illinois/1_June_2009 1]
| [http://2009.igem.org/wiki/index.php?title=Illinois/2_June_2009&action=edit 2]
| [http://2009.igem.org/wiki/index.php?title=Illinois/3_June_2009&action=edit 3]
| [http://2009.igem.org/wiki/index.php?title=Illinois/4_June_2009&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Illinois/5_June_2009&action=edit 5]
| [http://2009.igem.org/wiki/index.php?title=Illinois/6_June_2009&action=edit 6]
| [http://2009.igem.org/wiki/index.php?title=Illinois/7_June_2009&action=edit 7]
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| [http://2009.igem.org/wiki/index.php?title=Illinois/8_June_2009&action=edit 8]
| [http://2009.igem.org/wiki/index.php?title=Illinois/9_June_2009&action=edit 9]
| [http://2009.igem.org/wiki/index.php?title=Illinois/10_June_2009&action=edit 10]
| [http://2009.igem.org/wiki/index.php?title=Illinois/11_June_2009&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Illinois/12_June_2009&action=edit 12]
| [http://2009.igem.org/wiki/index.php?title=Illinois/13_June_2009&action=edit 13]
| [http://2009.igem.org/wiki/index.php?title=Illinois/14_June_2009&action=edit 14]
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| [http://2009.igem.org/Illinois/15_June_2009 15]
| [http://2009.igem.org/Illinois/16_June_2009 16]
| [http://2009.igem.org/Illinois/17_June_2009 17]
| [http://2009.igem.org/Illinois/18_June_2009 18]
| [http://2009.igem.org/Illinois/19_June_2009 19]
| [http://2009.igem.org/wiki/index.php?title=Illinois/20_June_2009&action=edit 20]
| [http://2009.igem.org/wiki/index.php?title=Illinois/21_June_2009&action=edit 21]
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| [http://2009.igem.org/wiki/index.php?title=Illinois/22_June_2009&action=edit 22]
| [http://2009.igem.org/Illinois/23_June_2009 23]
| [http://2009.igem.org/Illinois/24_June_2009 24]
| [http://2009.igem.org/Illinois/25_June_2009 25]
| [http://2009.igem.org/Illinois/26_June_2009 26]
| [http://2009.igem.org/wiki/index.php?title=Illinois/27_June_2009&action=edit 27]
| [http://2009.igem.org/wiki/index.php?title=Illinois/28_June_2009&action=edit 28]
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| [http://2009.igem.org/Illinois/29_June_2009 29]
| [http://2009.igem.org/Illinois/30_June_2009 30]
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| July
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| M | T | W | T | F | S | S
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| [http://2009.igem.org/Illinois/1_July_2009 1]
| [http://2009.igem.org/Illinois/2_July_2009 2]
| [http://2009.igem.org/Illinois/3_July_2009 3]
| [http://2009.igem.org/wiki/index.php?title=Illinois/4_July_2009&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Illinois/5_July_2009&action=edit 5]
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| [http://2009.igem.org/Illinois/6_July_2009 6]
| [http://2009.igem.org/Illinois/7_July_2009 7]
| [http://2009.igem.org/wiki/index.php?title=Illinois/8_July_2009&action=edit 8]
| [http://2009.igem.org/Illinois/9_July_2009 9]
| [http://2009.igem.org/Illinois/10_July_2009 10]
| [http://2009.igem.org/wiki/index.php?title=Illinois/11_July_2009&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Illinois/12_July_2009&action=edit 12]
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| [http://2009.igem.org/Illinois/13_July_2009 13]
| [http://2009.igem.org/Illinois/14_July_2009 14]
| [http://2009.igem.org/Illinois/15_July_2009 15]
| [http://2009.igem.org/Illinois/16_July_2009 16]
| [http://2009.igem.org/Illinois/17_July_2009 17]
| [http://2009.igem.org/wiki/index.php?title=Illinois/18_July_2009&action=edit 18]
| [http://2009.igem.org/wiki/index.php?title=Illinois/19_July_2009&action=edit 19]
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| [http://2009.igem.org/Illinois/20_July_2009 20]
| [http://2009.igem.org/Illinois/21_July_2009 21]
| [http://2009.igem.org/Illinois/22_July_2009 22]
| [http://2009.igem.org/Illinois/23_July_2009 23]
| [http://2009.igem.org/Illinois/24_July_2009 24]
| [http://2009.igem.org/wiki/index.php?title=Illinois/25_July_2009&action=edit 25]
| [http://2009.igem.org/wiki/index.php?title=Illinois/26_July_2009&action=edit 26]
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| [http://2009.igem.org/wiki/index.php?title=Illinois/27_July_2009&action=edit 27]
| [http://2009.igem.org/wiki/index.php?title=Illinois/28_July_2009&action=edit 28]
| [http://2009.igem.org/wiki/index.php?title=Illinois/29_July_2009&action=edit 29]
| [http://2009.igem.org/wiki/index.php?title=Illinois/30_July_2009&action=edit 30]
| [http://2009.igem.org/wiki/index.php?title=Illinois/31_July_2009&action=edit 31]
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| August
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| [http://2009.igem.org/wiki/index.php?title=Illinois/1_August_2009&action=edit 1]
| [http://2009.igem.org/wiki/index.php?title=Illinois/2_August_2009&action=edit 2]
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| [http://2009.igem.org/wiki/index.php?title=Illinois/3_August_2009&action=edit 3]
| [http://2009.igem.org/wiki/index.php?title=Illinois/4_August_2009&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Illinois/5_August_2009&action=edit 5]
| [http://2009.igem.org/wiki/index.php?title=Illinois/6_August_2009&action=edit 6]
| [http://2009.igem.org/wiki/index.php?title=Illinois/7_August_2009&action=edit 7]
| [http://2009.igem.org/wiki/index.php?title=Illinois/8_August_2009&action=edit 8]
| [http://2009.igem.org/wiki/index.php?title=Illinois/9_August_2009&action=edit 9]
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| [http://2009.igem.org/wiki/index.php?title=Illinois/10_August_2009&action=edit 10]
| [http://2009.igem.org/wiki/index.php?title=Illinois/11_August_2009&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Illinois/12_August_2009&action=edit 12]
| [http://2009.igem.org/wiki/index.php?title=Illinois/13_August_2009&action=edit 13]
| [http://2009.igem.org/wiki/index.php?title=Illinois/14_August_2009&action=edit 14]
| [http://2009.igem.org/wiki/index.php?title=Illinois/15_August_2009&action=edit 15]
| [http://2009.igem.org/wiki/index.php?title=Illinois/16_August_2009&action=edit 16]
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| [http://2009.igem.org/wiki/index.php?title=Illinois/17_August_2009&action=edit 17]
| [http://2009.igem.org/wiki/index.php?title=Illinois/18_August_2009&action=edit 18]
| [http://2009.igem.org/wiki/index.php?title=Illinois/19_August_2009&action=edit 19]
| [http://2009.igem.org/wiki/index.php?title=Illinois/20_August_2009&action=edit 20]
| [http://2009.igem.org/wiki/index.php?title=Illinois/21_August_2009&action=edit 21]
| [http://2009.igem.org/wiki/index.php?title=Illinois/22_August_2009&action=edit 22]
| [http://2009.igem.org/wiki/index.php?title=Illinois/23_August_2009&action=edit 23]
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| [http://2009.igem.org/wiki/index.php?title=Illinois/24_August_2009&action=edit 24]
| [http://2009.igem.org/wiki/index.php?title=Illinois/25_August_2009&action=edit 25]
| [http://2009.igem.org/wiki/index.php?title=Illinois/26_August_2009&action=edit 26]
| [http://2009.igem.org/wiki/index.php?title=Illinois/27_August_2009&action=edit 27]
| [http://2009.igem.org/wiki/index.php?title=Illinois/28_August_2009&action=edit 28]
| [http://2009.igem.org/wiki/index.php?title=Illinois/29_August_2009&action=edit 29]
| [http://2009.igem.org/wiki/index.php?title=Illinois/30_August_2009&action=edit 30]
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| [http://2009.igem.org/wiki/index.php?title=Illinois/31_August_2009&action=edit 31]
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July 14
Hybrid Promoter
In lab we performed a miniprep of the inoculated colony from yesterday to isolate the DNA. It took some time to get access to the centrifuge as it was in use for much of the morning.
At the meeting with the professors they suggested that we take a look at the availabe GFP generator biobricks in the registry. It was also suggested that we would need DH5 alpha Z E. Coli cells if we are to use the lac promoter. It was also mentioned that about two plasmids transformed into a cell was standard and that we could probably get three.
Modeling
We miniprepped our transformed colonies to obtain DNA for BBa_K113009 and BBa_I0500. We also ran a gel on our PCR reactions on the pSB3K3 plasmid backbone. Both reactions yielded ~2kbp bands, when they were expected to be at 2.75kbp. We have decided to use this DNA anyway, since this is the second time bands have shown up at 2kbp.
We planned on digesting our DNA overnight, but our DNA concentrations were too low: between 5 and 30 ng/μL per sample. We have to find out what we should do to compensate for low concentrations.
Back to Notebook page
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