Team:Paris/Protocols

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Protocols

iGEM paris 2007 protocols link :


content :

  • [http://parts.mit.edu/igem07/index.php/Paris/PROTOCOLS#Growing_bacteria_in_liquid_medium | Growing_bacteria_in_liquid_medium]
  • Preparing growth media
    • exemple: Making 10 petri dish (LB+erythromycin+citrate+DAP), Solid M9 Minimum Medium, preparation of agarosis gel
  • Chemical transformation
  • Glycerol Stock
  • Recombineering/Lambda red-mediated gene replacement
  • Miniprep
  • Fluorescent single cells visualisation
  • Digestion reactions

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