"
Virginia Commonwealth/23 July 2009
From 2009.igem.org
Maria and Afton
- Samples from cryogenic stocks were grown up in LB:
- DB 3.1 (2 vials)
- Samples from cryogenic stocks were grown up in LB + Amp:
- pSB4C5
- Samples from cryogenic stocks were grown up in LB + Cm:
| Promoter | RET | Growth Observation | |
|---|---|---|---|
| J23100 | E0240 | n/a | |
| J23101 | E0240 | ||
| J23101 | J06702 | ||
| J23102 | E0240 | ||
| J23102 | J06702 | ||
| J23103 | E0240 | ||
| J23103 | J06702 | ||
| J23104 | E0240 | ||
| J23104 | J06702 | ||
| J23105 | E0240 | ||
| J23105 | J06702 | ||
| J23106 | E0240 | ||
| J23106 | J06702 | ||
| J23107 | E0240 | ||
| J23107 | J06702 | ||
| J23110 | E0240 | ||
| J23110 | J06702 | ||
| J23113 | E0240 | ||
| J23113 | J06702 | ||
| J23116 | E0240 | ||
| J23116 | J06702 | ||
| R0011 | E0240 | ||
| R0011 | J06702 | ||
| R0040 | E0240 | ||
| R0040 | J06702 |
- Transformation of electrocompetent E. Coli stock and BioBrick with promoter J23100 and RET J06702
| Name | Type | Plate | Purpose | Growth Observation | |
|---|---|---|---|---|---|
| NEB 10 beta | seed stock | LB | make more seed stock | lawn | |
| NEB 10 beta | electro comp. | LB | (+) Control/ make more stock | lawn | |
| NEB 10 beta | electro comp. | LB | (+) Control shocked | lawn | |
| DB 3.1 | electro comp. | LB | (+) Control shocked | lawn | |
| DB 3.1 | electro comp. | LB | (+) Control/make more stock | lawn | |
| NEB 10 beta | electro comp. | Cm | (-) Control | no growth | |
| NEB 10 beta | electro comp. | Cm | J23100 w/ J06702 | 6 small colonies |
Trentay 14:18, 23 July 2009 (UTC)
