Team:Virginia/27 July 2009

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Overnight Results

  • All platings blank again

Today

  • PCR to add HA tag to ABP
    • 1ul of ABP DNA @ 422ng/ul
    • 3 different MgCl2 concentrations: 1, 2, 4 mM
    • Control w/o ABP DNA
    • 2 cycles with 58C annealing temp, then 28 cycles with 65C annealing temp
    • 74C extension step, 10 sec
    • Vgem072709.jpg
    • Ladder, No template DNA, Template DNA & 1mM MgCl2,...& 2mM MgCl2,...& 4mM MgCl2
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